Genomic counter-stress changes induced by the relaxation response

Jeffrey A. Dusek, Hasan H. Otu, Ann L. Wohlhueter, Manoj Bhasin, Luiz F. Zerbini, Marie G. Joseph, Herbert Benson, Towia A. Libermann

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Background: Mind-body practices that elicit the relaxation response (RR) have been used worldwide for millennia to prevent and treat disease. The RR is characterized by decreased oxygen consumption, increased exhaled nitric oxide, and reduced psychological distress. It is believed to be the counterpart of the stress response that exhibits a distinct pattern of physiology and transcriptional profile. We hypothesized that RR elicitation results in characteristic gene expression changes that can be used to measure physiological responses elicited by the RR in an unbiased fashion. Methods/Principal Findings: We assessed whole blood transcriptional profiles in 19 healthy, long-term practitioners of daily RR practice (group M), 19 healthy controls (group N1), and 20 N1 individuals who completed 8 weeks of RR training (group N2). 2209 genes were differentially expressed in group M relative to group N1 (p<0.05) and 1561 genes in group N2 compared to group N1 (p<0.05). Importantly, 433 (p<10-10) of 2209 and 1561 differentially expressed genes were shared among long-term (M) and short-term practitioners (N2). Gene ontology and gene set enrichment analyses revealed significant alterations in cellular metabolism, oxidative phosphorylation, generation of reactive oxygen species and response to oxidative stress in long-term and short-term practitioners of daily RR practice that may counteract cellular damage related to chronic psychological stress. A significant number of genes and pathways were confirmed in an independent validation set containing 5 N1 controls, 5 N2 short-term and 6 M long-term practitioners. Conclusions/Significance. This study provides the first compelling evidence that the RR elicits specific gene expression changes in short-term and long-term practitioners. Our results suggest consistent and constitutive changes in gene expression resulting from RR may relate to long term physiological effects. Our study may stimulate new investigations into applying transcriptional profiling for accurately measuring RR and stress related responses in multiple disease settings.

Original languageEnglish (US)
Article numbere2576
JournalPloS one
Volume3
Issue number7
DOIs
StatePublished - Jul 2 2008

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Genes
genomics
Gene expression
Gene Expression
genes
gene expression
Gene Ontology
Oxidative Phosphorylation
Oxidative stress
Psychological Stress
oxidative phosphorylation
Oxygen Consumption
Physiology
distress
Reactive Oxygen Species
Nitric Oxide
Metabolism
Oxidative Stress
oxygen consumption
nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Dusek, J. A., Otu, H. H., Wohlhueter, A. L., Bhasin, M., Zerbini, L. F., Joseph, M. G., ... Libermann, T. A. (2008). Genomic counter-stress changes induced by the relaxation response. PloS one, 3(7), [e2576]. https://doi.org/10.1371/journal.pone.0002576

Genomic counter-stress changes induced by the relaxation response. / Dusek, Jeffrey A.; Otu, Hasan H.; Wohlhueter, Ann L.; Bhasin, Manoj; Zerbini, Luiz F.; Joseph, Marie G.; Benson, Herbert; Libermann, Towia A.

In: PloS one, Vol. 3, No. 7, e2576, 02.07.2008.

Research output: Contribution to journalArticle

Dusek, JA, Otu, HH, Wohlhueter, AL, Bhasin, M, Zerbini, LF, Joseph, MG, Benson, H & Libermann, TA 2008, 'Genomic counter-stress changes induced by the relaxation response', PloS one, vol. 3, no. 7, e2576. https://doi.org/10.1371/journal.pone.0002576
Dusek JA, Otu HH, Wohlhueter AL, Bhasin M, Zerbini LF, Joseph MG et al. Genomic counter-stress changes induced by the relaxation response. PloS one. 2008 Jul 2;3(7). e2576. https://doi.org/10.1371/journal.pone.0002576
Dusek, Jeffrey A. ; Otu, Hasan H. ; Wohlhueter, Ann L. ; Bhasin, Manoj ; Zerbini, Luiz F. ; Joseph, Marie G. ; Benson, Herbert ; Libermann, Towia A. / Genomic counter-stress changes induced by the relaxation response. In: PloS one. 2008 ; Vol. 3, No. 7.
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