Genomic and proteomic microglial profiling: Pathways for neuroprotective inflammatory responses following nerve fragment clearance and activation

Jason G. Glanzer, Yoshimi Enose, Tong Wang, Irena Kadiu, Nan Gong, Wojciech Rozek, Jianuo Liu, Joshua D. Schlautman, Pawel S Ciborowski, Mark P. Thomas, Howard Eliot Gendelman

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Microglia, a primary immune effector cell of the central nervous system (CNS) affects homeostatic, neuroprotective, regenerative and degenerative outcomes in health and disease. Despite these broad neuroimmune activities linked to specific environmental cues, a precise cellular genetic profile for microglia in the context of disease and repair has not been elucidated. To this end we used nucleic acid microarrays, proteomics, immunochemical and histochemical tests to profile microglia in neuroprotective immune responses. Optic and sciatic nerve (ON and SN) fragments were used to stimulate microglia in order to reflect immune consequences of nervous system injury. Lipopolysaccharide and latex beads-induced microglial activation served as positive controls. Cytosolic and secreted proteins were profiled by surface enhanced laser desorption ionization-time of flight (SELDI-TOF) ProteinChip®, 1D and 2D difference gel electrophoresis. Proteins were identified by peptide sequencing with tandem mass spectrometry, ELISA and western blot tests. Temporal expression of pro-inflammatory cytokines, antioxidants, neurotrophins, and lysosomal enzyme expression provided, for the first time, a unique profile of secreted microglia proteins with neuroregulatory functions. Most importantly, this molecular and biochemical signature supports a broad range of microglial functions for debris clearance and promotion of neural repair after injury.

Original languageEnglish (US)
Pages (from-to)627-645
Number of pages19
JournalJournal of Neurochemistry
Volume102
Issue number3
DOIs
StatePublished - Aug 1 2007

Fingerprint

Microglia
Proteomics
Chemical activation
Neurology
Repair
Nervous System Trauma
Protein Array Analysis
Proteins
Latex
Electrophoresis, Gel, Two-Dimensional
Nerve Growth Factors
Sciatic Nerve
Microarrays
Optic Nerve
Tandem Mass Spectrometry
Electrophoresis
Microspheres
Debris
Nucleic Acids
Ionization

Keywords

  • Cathepsins
  • Microglia
  • NFκB
  • Neurotrophins
  • Optic nerve
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Genomic and proteomic microglial profiling : Pathways for neuroprotective inflammatory responses following nerve fragment clearance and activation. / Glanzer, Jason G.; Enose, Yoshimi; Wang, Tong; Kadiu, Irena; Gong, Nan; Rozek, Wojciech; Liu, Jianuo; Schlautman, Joshua D.; Ciborowski, Pawel S; Thomas, Mark P.; Gendelman, Howard Eliot.

In: Journal of Neurochemistry, Vol. 102, No. 3, 01.08.2007, p. 627-645.

Research output: Contribution to journalArticle

Glanzer, Jason G. ; Enose, Yoshimi ; Wang, Tong ; Kadiu, Irena ; Gong, Nan ; Rozek, Wojciech ; Liu, Jianuo ; Schlautman, Joshua D. ; Ciborowski, Pawel S ; Thomas, Mark P. ; Gendelman, Howard Eliot. / Genomic and proteomic microglial profiling : Pathways for neuroprotective inflammatory responses following nerve fragment clearance and activation. In: Journal of Neurochemistry. 2007 ; Vol. 102, No. 3. pp. 627-645.
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