Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.

Elena I. Stepchenkova, Stanislav G. Kozmin, Vladimir V. Alenin, Youri I. Pavlov

Research output: Contribution to journalArticle

Abstract

N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast. We screened the library of yeast deletion mutants for sensitivity to the toxic and mutagenic action of HAP and AHA. We identified novel genes involved in the genetic control of base analogs sensitivity, including genes controlling purine metabolism, cytoskeleton organization, and amino acid metabolism. We developed a method for screening the yeast deletion library for sensitivity to the mutagenic and toxic action of base analogs and identified 16 novel genes controlling pathways of protection from HAP. Three of them also protect from AHA.

Original languageEnglish (US)
Article number31
JournalBMC Genetics
Volume6
StatePublished - 2005
Externally publishedYes

Fingerprint

Gene Deletion
Yeasts
Genome
Toxic Actions
Libraries
Deoxyribonuclease (Pyrimidine Dimer)
Deoxyribonucleosides
Genes
Purines
Deoxyribonuclease I
Mutagens
Eukaryota
Cytoskeleton
Base Pairing
DNA Repair
Saccharomyces cerevisiae
Escherichia coli
Amino Acids
purine

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast. / Stepchenkova, Elena I.; Kozmin, Stanislav G.; Alenin, Vladimir V.; Pavlov, Youri I.

In: BMC Genetics, Vol. 6, 31, 2005.

Research output: Contribution to journalArticle

@article{ea4d7b8b7f8f4d039c1bceac443c5e34,
title = "Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.",
abstract = "N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast. We screened the library of yeast deletion mutants for sensitivity to the toxic and mutagenic action of HAP and AHA. We identified novel genes involved in the genetic control of base analogs sensitivity, including genes controlling purine metabolism, cytoskeleton organization, and amino acid metabolism. We developed a method for screening the yeast deletion library for sensitivity to the mutagenic and toxic action of base analogs and identified 16 novel genes controlling pathways of protection from HAP. Three of them also protect from AHA.",
author = "Stepchenkova, {Elena I.} and Kozmin, {Stanislav G.} and Alenin, {Vladimir V.} and Pavlov, {Youri I.}",
year = "2005",
language = "English (US)",
volume = "6",
journal = "BMC Genetics",
issn = "1471-2156",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.

AU - Stepchenkova, Elena I.

AU - Kozmin, Stanislav G.

AU - Alenin, Vladimir V.

AU - Pavlov, Youri I.

PY - 2005

Y1 - 2005

N2 - N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast. We screened the library of yeast deletion mutants for sensitivity to the toxic and mutagenic action of HAP and AHA. We identified novel genes involved in the genetic control of base analogs sensitivity, including genes controlling purine metabolism, cytoskeleton organization, and amino acid metabolism. We developed a method for screening the yeast deletion library for sensitivity to the mutagenic and toxic action of base analogs and identified 16 novel genes controlling pathways of protection from HAP. Three of them also protect from AHA.

AB - N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast. We screened the library of yeast deletion mutants for sensitivity to the toxic and mutagenic action of HAP and AHA. We identified novel genes involved in the genetic control of base analogs sensitivity, including genes controlling purine metabolism, cytoskeleton organization, and amino acid metabolism. We developed a method for screening the yeast deletion library for sensitivity to the mutagenic and toxic action of base analogs and identified 16 novel genes controlling pathways of protection from HAP. Three of them also protect from AHA.

UR - http://www.scopus.com/inward/record.url?scp=84872258078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872258078&partnerID=8YFLogxK

M3 - Article

C2 - 15932646

VL - 6

JO - BMC Genetics

JF - BMC Genetics

SN - 1471-2156

M1 - 31

ER -