Genome wide DNA-profiling of HIV-related B-cell lymphomas

Daniela Capello, Marta Scandurra, Giulia Poretti, Paola M V Rancoita, Michael Mian, Annunziata Gloghini, Clara Deambrogi, Maurizio Martini, Davide Rossi, Timothy Charles Greiner, Wing C. Chan, Maurilio Ponzoni, Santiago M. Moreno, Miguel A. Piris, Vincenzo Canzonieri, Michele Spina, Umberto Tirelli, Giorgio Inghirami, Andrea Rinaldi, Emanuele Zucca & 7 others Riccardo D. Favera, Franco Cavalli, Luigi Maria Larocca, Ivo Kwee, Antonino Carbone, Gianluca Gaidano, Francesco Bertoni

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Non-Hodgkin lymphomas (NHL) represent a frequent complication of human immunodeficiency virus (HIV) infection. To elucidate HIV-NHL pathogenesis, we performed a genome-wide DNA profiling based on a single nucleotide polymorphism-based microarray comparative genomic hybridization in 57 HIV-lymphomas and, for comparison, in 105 immunocompetent diffuse large B-cell lymphomas (IC-DLBCL). Genomic complexity varied across HIV-NHL subtypes. HIV-Burkitt lymphoma showed a significantly lower number of lesions than HIV-DLBCL (P = 0·032), whereas the median number of copy number changes was significantly higher in Epstein-Barr virus negative (EBV-) HIV-DLBCL (42·5, range 8-153) compared to EBV+ cases (22; range 3-41; P = 0·029). Compared to IC-DLBCL, HIV-DLBCL displayed a distinct genomic profile with no gains of 18q and specific genetic lesions. Fragile sites-associated genes, including FHIT (FRA3B), WWOX (FRA16D), DCC (FRA18B) and PARK2 (FRA6E) were frequently inactivated in HIV-NHL by interstitial deletions, and a significantly higher prevalence of FHIT alterations was observed in HIV-DLBCL compared to IC-DLBCL. The same genes involved by fragile site deletions were also frequently affected by aberrant methylation of regulative regions.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalBritish Journal of Haematology
Volume148
Issue number2
DOIs
StatePublished - Jan 1 2010

Fingerprint

DNA Fingerprinting
B-Cell Lymphoma
HIV
Genome
Non-Hodgkin's Lymphoma
Lymphoma, Large B-Cell, Diffuse
Human Herpesvirus 4
Comparative Genomic Hybridization
Burkitt Lymphoma
Virus Diseases
Methylation
Genes
Single Nucleotide Polymorphism
Lymphoma

Keywords

  • Acquired immunodeficiency syndrome
  • Affymetrix
  • Diffuse large B-cell lymphoma
  • Fragile Histidine Triad
  • WW domain-containing Oxidoreductase

ASJC Scopus subject areas

  • Hematology

Cite this

Capello, D., Scandurra, M., Poretti, G., Rancoita, P. M. V., Mian, M., Gloghini, A., ... Bertoni, F. (2010). Genome wide DNA-profiling of HIV-related B-cell lymphomas. British Journal of Haematology, 148(2), 245-255. https://doi.org/10.1111/j.1365-2141.2009.07943.x

Genome wide DNA-profiling of HIV-related B-cell lymphomas. / Capello, Daniela; Scandurra, Marta; Poretti, Giulia; Rancoita, Paola M V; Mian, Michael; Gloghini, Annunziata; Deambrogi, Clara; Martini, Maurizio; Rossi, Davide; Greiner, Timothy Charles; Chan, Wing C.; Ponzoni, Maurilio; Moreno, Santiago M.; Piris, Miguel A.; Canzonieri, Vincenzo; Spina, Michele; Tirelli, Umberto; Inghirami, Giorgio; Rinaldi, Andrea; Zucca, Emanuele; Favera, Riccardo D.; Cavalli, Franco; Larocca, Luigi Maria; Kwee, Ivo; Carbone, Antonino; Gaidano, Gianluca; Bertoni, Francesco.

In: British Journal of Haematology, Vol. 148, No. 2, 01.01.2010, p. 245-255.

Research output: Contribution to journalArticle

Capello, D, Scandurra, M, Poretti, G, Rancoita, PMV, Mian, M, Gloghini, A, Deambrogi, C, Martini, M, Rossi, D, Greiner, TC, Chan, WC, Ponzoni, M, Moreno, SM, Piris, MA, Canzonieri, V, Spina, M, Tirelli, U, Inghirami, G, Rinaldi, A, Zucca, E, Favera, RD, Cavalli, F, Larocca, LM, Kwee, I, Carbone, A, Gaidano, G & Bertoni, F 2010, 'Genome wide DNA-profiling of HIV-related B-cell lymphomas', British Journal of Haematology, vol. 148, no. 2, pp. 245-255. https://doi.org/10.1111/j.1365-2141.2009.07943.x
Capello D, Scandurra M, Poretti G, Rancoita PMV, Mian M, Gloghini A et al. Genome wide DNA-profiling of HIV-related B-cell lymphomas. British Journal of Haematology. 2010 Jan 1;148(2):245-255. https://doi.org/10.1111/j.1365-2141.2009.07943.x
Capello, Daniela ; Scandurra, Marta ; Poretti, Giulia ; Rancoita, Paola M V ; Mian, Michael ; Gloghini, Annunziata ; Deambrogi, Clara ; Martini, Maurizio ; Rossi, Davide ; Greiner, Timothy Charles ; Chan, Wing C. ; Ponzoni, Maurilio ; Moreno, Santiago M. ; Piris, Miguel A. ; Canzonieri, Vincenzo ; Spina, Michele ; Tirelli, Umberto ; Inghirami, Giorgio ; Rinaldi, Andrea ; Zucca, Emanuele ; Favera, Riccardo D. ; Cavalli, Franco ; Larocca, Luigi Maria ; Kwee, Ivo ; Carbone, Antonino ; Gaidano, Gianluca ; Bertoni, Francesco. / Genome wide DNA-profiling of HIV-related B-cell lymphomas. In: British Journal of Haematology. 2010 ; Vol. 148, No. 2. pp. 245-255.
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