Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4

Benjamin Janto, Azad Ahmed, Masahiro Ito, Jun Liu, David B. Hicks, Sarah Pagni, Oliver J. Fackelmayer, Terry Ann Smith, Joshua Earl, Liam D.H. Elbourne, Karl Hassan, Ian T. Paulsen, Anne Brit Kolstø, Nicolas J. Tourasse, Garth D. Ehrlich, Robert Boissy, D. Mack Ivey, Gang Li, Yanfen Xue, Yanhe MaFen Z. Hu, Terry A. Krulwich

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.

Original languageEnglish (US)
Pages (from-to)3289-3309
Number of pages21
JournalEnvironmental microbiology
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Bacillus pseudofirmus
homeostasis
plasmid
Bacillus
genome
Genome
bioenergetics
energy
viability
environmental conditions
sodium
protein
gene
plasmids
Plasmids
Homeostasis
Regulator Genes
regulator genes
energy metabolism
Cell Wall

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4. / Janto, Benjamin; Ahmed, Azad; Ito, Masahiro; Liu, Jun; Hicks, David B.; Pagni, Sarah; Fackelmayer, Oliver J.; Smith, Terry Ann; Earl, Joshua; Elbourne, Liam D.H.; Hassan, Karl; Paulsen, Ian T.; Kolstø, Anne Brit; Tourasse, Nicolas J.; Ehrlich, Garth D.; Boissy, Robert; Ivey, D. Mack; Li, Gang; Xue, Yanfen; Ma, Yanhe; Hu, Fen Z.; Krulwich, Terry A.

In: Environmental microbiology, Vol. 13, No. 12, 01.12.2011, p. 3289-3309.

Research output: Contribution to journalArticle

Janto, B, Ahmed, A, Ito, M, Liu, J, Hicks, DB, Pagni, S, Fackelmayer, OJ, Smith, TA, Earl, J, Elbourne, LDH, Hassan, K, Paulsen, IT, Kolstø, AB, Tourasse, NJ, Ehrlich, GD, Boissy, R, Ivey, DM, Li, G, Xue, Y, Ma, Y, Hu, FZ & Krulwich, TA 2011, 'Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4', Environmental microbiology, vol. 13, no. 12, pp. 3289-3309. https://doi.org/10.1111/j.1462-2920.2011.02591.x
Janto, Benjamin ; Ahmed, Azad ; Ito, Masahiro ; Liu, Jun ; Hicks, David B. ; Pagni, Sarah ; Fackelmayer, Oliver J. ; Smith, Terry Ann ; Earl, Joshua ; Elbourne, Liam D.H. ; Hassan, Karl ; Paulsen, Ian T. ; Kolstø, Anne Brit ; Tourasse, Nicolas J. ; Ehrlich, Garth D. ; Boissy, Robert ; Ivey, D. Mack ; Li, Gang ; Xue, Yanfen ; Ma, Yanhe ; Hu, Fen Z. ; Krulwich, Terry A. / Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4. In: Environmental microbiology. 2011 ; Vol. 13, No. 12. pp. 3289-3309.
@article{8ccfa7212ea345f89f5b3ba1e7b44e80,
title = "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4",
abstract = "Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.",
author = "Benjamin Janto and Azad Ahmed and Masahiro Ito and Jun Liu and Hicks, {David B.} and Sarah Pagni and Fackelmayer, {Oliver J.} and Smith, {Terry Ann} and Joshua Earl and Elbourne, {Liam D.H.} and Karl Hassan and Paulsen, {Ian T.} and Kolst{\o}, {Anne Brit} and Tourasse, {Nicolas J.} and Ehrlich, {Garth D.} and Robert Boissy and Ivey, {D. Mack} and Gang Li and Yanfen Xue and Yanhe Ma and Hu, {Fen Z.} and Krulwich, {Terry A.}",
year = "2011",
month = "12",
day = "1",
doi = "10.1111/j.1462-2920.2011.02591.x",
language = "English (US)",
volume = "13",
pages = "3289--3309",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4

AU - Janto, Benjamin

AU - Ahmed, Azad

AU - Ito, Masahiro

AU - Liu, Jun

AU - Hicks, David B.

AU - Pagni, Sarah

AU - Fackelmayer, Oliver J.

AU - Smith, Terry Ann

AU - Earl, Joshua

AU - Elbourne, Liam D.H.

AU - Hassan, Karl

AU - Paulsen, Ian T.

AU - Kolstø, Anne Brit

AU - Tourasse, Nicolas J.

AU - Ehrlich, Garth D.

AU - Boissy, Robert

AU - Ivey, D. Mack

AU - Li, Gang

AU - Xue, Yanfen

AU - Ma, Yanhe

AU - Hu, Fen Z.

AU - Krulwich, Terry A.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.

AB - Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.

UR - http://www.scopus.com/inward/record.url?scp=82555205330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82555205330&partnerID=8YFLogxK

U2 - 10.1111/j.1462-2920.2011.02591.x

DO - 10.1111/j.1462-2920.2011.02591.x

M3 - Article

C2 - 21951522

AN - SCOPUS:82555205330

VL - 13

SP - 3289

EP - 3309

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 12

ER -