Genistein treatment duration effects biomarkers of cell motility in human prostate

Hu Zhang, Ryan Gordon, Wenqi Li, Ximing Yang, Abhinandan Pattanayak, Graham Fowler, Limin Zhang, William J. Catalona, Yongzeng Ding, Li Xu, Xiaoke Huang, Borko Jovanovic, David Lee Kelly, Haowen Jiang, Raymond Bergan

Research output: Contribution to journalArticle

Abstract

Background Long term dietary consumption of genistein by Chinese men is associated with decreased PCa metastasis and mortality. Short term treatment of US men with prostate cancer (PCa) with genistein decreases MMP-2 in prostate tissue. MEK4 regulates MMP-2 expression, drives PCa metastasis, and genistein inhibits MEK4, decreases MMP-2 expression and dietary dosing inhibits human PCa metastasis in mice. This study examines short- versus long-term treatment effects of genistein in humans and in vitro. Methods and findings US men with localized PCa were treated on a phase II trial with genistein (N = 14) versus not (N = 14) for one month prior to radical prostatectomy. Prostate epithelial cells were removed from fresh frozen tissue by laser capture microdissection, and the expression of 12,000 genes profiled. Genistein significantly altered the expression of four genes, three had established links to cancer cell motility and metastasis. Of these three, one was a non-coding transcript, and the other two were BASP1 and HCF2. Genistein increased BASP1 expression in humans, and its engineered over expression and knockdown demonstrated that it suppressed cell invasion in all six human prostate cell lines examined. Genistein decreased HCF2 expression in humans, and it was shown to increase cell invasion in all cell lines examined. The expression of MMP-2, MEK4 and BASP1 was then measured in formalin fixed prostate tissue from N = 38 Chinese men living in China and N = 41 US men living in the US, both cohorts with localized PCa. MMP-2 was 52% higher in Chinese compared to US tissue (P < 0.0001), MEK4 was 48% lower (P < 0.0001), and BASP1 was unaltered. Treatment of PC3 human PCa cells in vitro for up to 8 weeks demonstrated that short term genistein treatment decreased MMP-2, while long term treatment increased it, both changes being significant (P<0.05) compared to untreated control cells. Long term genistein-treated cells retained their responsiveness to genistein’s anti-motility effect. Conclusions Genistein inhibits pathways in human prostate that drive transformation to a lethal high motility phenotype. Long term treatment induces compensatory changes in biomarkers of efficacy. The current strategy of using such biomarkers after short term intervention as go/ no-go determinants in early phase chemoprevention trials should be carefully examined.

Original languageEnglish (US)
Article numbere0214078
JournalPloS one
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2019

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Genistein
genistein
Biomarkers
cell movement
Cell Movement
Prostate
biomarkers
prostatic neoplasms
gelatinase A
duration
Matrix Metalloproteinases
Prostatic Neoplasms
metastasis
Cells
Therapeutics
Tissue
Neoplasm Metastasis
cell invasion
Microdissection
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Zhang, H., Gordon, R., Li, W., Yang, X., Pattanayak, A., Fowler, G., ... Bergan, R. (2019). Genistein treatment duration effects biomarkers of cell motility in human prostate. PloS one, 14(3), [e0214078]. https://doi.org/10.1371/journal.pone.0214078

Genistein treatment duration effects biomarkers of cell motility in human prostate. / Zhang, Hu; Gordon, Ryan; Li, Wenqi; Yang, Ximing; Pattanayak, Abhinandan; Fowler, Graham; Zhang, Limin; Catalona, William J.; Ding, Yongzeng; Xu, Li; Huang, Xiaoke; Jovanovic, Borko; Kelly, David Lee; Jiang, Haowen; Bergan, Raymond.

In: PloS one, Vol. 14, No. 3, e0214078, 01.03.2019.

Research output: Contribution to journalArticle

Zhang, H, Gordon, R, Li, W, Yang, X, Pattanayak, A, Fowler, G, Zhang, L, Catalona, WJ, Ding, Y, Xu, L, Huang, X, Jovanovic, B, Kelly, DL, Jiang, H & Bergan, R 2019, 'Genistein treatment duration effects biomarkers of cell motility in human prostate', PloS one, vol. 14, no. 3, e0214078. https://doi.org/10.1371/journal.pone.0214078
Zhang H, Gordon R, Li W, Yang X, Pattanayak A, Fowler G et al. Genistein treatment duration effects biomarkers of cell motility in human prostate. PloS one. 2019 Mar 1;14(3). e0214078. https://doi.org/10.1371/journal.pone.0214078
Zhang, Hu ; Gordon, Ryan ; Li, Wenqi ; Yang, Ximing ; Pattanayak, Abhinandan ; Fowler, Graham ; Zhang, Limin ; Catalona, William J. ; Ding, Yongzeng ; Xu, Li ; Huang, Xiaoke ; Jovanovic, Borko ; Kelly, David Lee ; Jiang, Haowen ; Bergan, Raymond. / Genistein treatment duration effects biomarkers of cell motility in human prostate. In: PloS one. 2019 ; Vol. 14, No. 3.
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abstract = "Background Long term dietary consumption of genistein by Chinese men is associated with decreased PCa metastasis and mortality. Short term treatment of US men with prostate cancer (PCa) with genistein decreases MMP-2 in prostate tissue. MEK4 regulates MMP-2 expression, drives PCa metastasis, and genistein inhibits MEK4, decreases MMP-2 expression and dietary dosing inhibits human PCa metastasis in mice. This study examines short- versus long-term treatment effects of genistein in humans and in vitro. Methods and findings US men with localized PCa were treated on a phase II trial with genistein (N = 14) versus not (N = 14) for one month prior to radical prostatectomy. Prostate epithelial cells were removed from fresh frozen tissue by laser capture microdissection, and the expression of 12,000 genes profiled. Genistein significantly altered the expression of four genes, three had established links to cancer cell motility and metastasis. Of these three, one was a non-coding transcript, and the other two were BASP1 and HCF2. Genistein increased BASP1 expression in humans, and its engineered over expression and knockdown demonstrated that it suppressed cell invasion in all six human prostate cell lines examined. Genistein decreased HCF2 expression in humans, and it was shown to increase cell invasion in all cell lines examined. The expression of MMP-2, MEK4 and BASP1 was then measured in formalin fixed prostate tissue from N = 38 Chinese men living in China and N = 41 US men living in the US, both cohorts with localized PCa. MMP-2 was 52{\%} higher in Chinese compared to US tissue (P < 0.0001), MEK4 was 48{\%} lower (P < 0.0001), and BASP1 was unaltered. Treatment of PC3 human PCa cells in vitro for up to 8 weeks demonstrated that short term genistein treatment decreased MMP-2, while long term treatment increased it, both changes being significant (P<0.05) compared to untreated control cells. Long term genistein-treated cells retained their responsiveness to genistein’s anti-motility effect. Conclusions Genistein inhibits pathways in human prostate that drive transformation to a lethal high motility phenotype. Long term treatment induces compensatory changes in biomarkers of efficacy. The current strategy of using such biomarkers after short term intervention as go/ no-go determinants in early phase chemoprevention trials should be carefully examined.",
author = "Hu Zhang and Ryan Gordon and Wenqi Li and Ximing Yang and Abhinandan Pattanayak and Graham Fowler and Limin Zhang and Catalona, {William J.} and Yongzeng Ding and Li Xu and Xiaoke Huang and Borko Jovanovic and Kelly, {David Lee} and Haowen Jiang and Raymond Bergan",
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AU - Zhang, Hu

AU - Gordon, Ryan

AU - Li, Wenqi

AU - Yang, Ximing

AU - Pattanayak, Abhinandan

AU - Fowler, Graham

AU - Zhang, Limin

AU - Catalona, William J.

AU - Ding, Yongzeng

AU - Xu, Li

AU - Huang, Xiaoke

AU - Jovanovic, Borko

AU - Kelly, David Lee

AU - Jiang, Haowen

AU - Bergan, Raymond

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Background Long term dietary consumption of genistein by Chinese men is associated with decreased PCa metastasis and mortality. Short term treatment of US men with prostate cancer (PCa) with genistein decreases MMP-2 in prostate tissue. MEK4 regulates MMP-2 expression, drives PCa metastasis, and genistein inhibits MEK4, decreases MMP-2 expression and dietary dosing inhibits human PCa metastasis in mice. This study examines short- versus long-term treatment effects of genistein in humans and in vitro. Methods and findings US men with localized PCa were treated on a phase II trial with genistein (N = 14) versus not (N = 14) for one month prior to radical prostatectomy. Prostate epithelial cells were removed from fresh frozen tissue by laser capture microdissection, and the expression of 12,000 genes profiled. Genistein significantly altered the expression of four genes, three had established links to cancer cell motility and metastasis. Of these three, one was a non-coding transcript, and the other two were BASP1 and HCF2. Genistein increased BASP1 expression in humans, and its engineered over expression and knockdown demonstrated that it suppressed cell invasion in all six human prostate cell lines examined. Genistein decreased HCF2 expression in humans, and it was shown to increase cell invasion in all cell lines examined. The expression of MMP-2, MEK4 and BASP1 was then measured in formalin fixed prostate tissue from N = 38 Chinese men living in China and N = 41 US men living in the US, both cohorts with localized PCa. MMP-2 was 52% higher in Chinese compared to US tissue (P < 0.0001), MEK4 was 48% lower (P < 0.0001), and BASP1 was unaltered. Treatment of PC3 human PCa cells in vitro for up to 8 weeks demonstrated that short term genistein treatment decreased MMP-2, while long term treatment increased it, both changes being significant (P<0.05) compared to untreated control cells. Long term genistein-treated cells retained their responsiveness to genistein’s anti-motility effect. Conclusions Genistein inhibits pathways in human prostate that drive transformation to a lethal high motility phenotype. Long term treatment induces compensatory changes in biomarkers of efficacy. The current strategy of using such biomarkers after short term intervention as go/ no-go determinants in early phase chemoprevention trials should be carefully examined.

AB - Background Long term dietary consumption of genistein by Chinese men is associated with decreased PCa metastasis and mortality. Short term treatment of US men with prostate cancer (PCa) with genistein decreases MMP-2 in prostate tissue. MEK4 regulates MMP-2 expression, drives PCa metastasis, and genistein inhibits MEK4, decreases MMP-2 expression and dietary dosing inhibits human PCa metastasis in mice. This study examines short- versus long-term treatment effects of genistein in humans and in vitro. Methods and findings US men with localized PCa were treated on a phase II trial with genistein (N = 14) versus not (N = 14) for one month prior to radical prostatectomy. Prostate epithelial cells were removed from fresh frozen tissue by laser capture microdissection, and the expression of 12,000 genes profiled. Genistein significantly altered the expression of four genes, three had established links to cancer cell motility and metastasis. Of these three, one was a non-coding transcript, and the other two were BASP1 and HCF2. Genistein increased BASP1 expression in humans, and its engineered over expression and knockdown demonstrated that it suppressed cell invasion in all six human prostate cell lines examined. Genistein decreased HCF2 expression in humans, and it was shown to increase cell invasion in all cell lines examined. The expression of MMP-2, MEK4 and BASP1 was then measured in formalin fixed prostate tissue from N = 38 Chinese men living in China and N = 41 US men living in the US, both cohorts with localized PCa. MMP-2 was 52% higher in Chinese compared to US tissue (P < 0.0001), MEK4 was 48% lower (P < 0.0001), and BASP1 was unaltered. Treatment of PC3 human PCa cells in vitro for up to 8 weeks demonstrated that short term genistein treatment decreased MMP-2, while long term treatment increased it, both changes being significant (P<0.05) compared to untreated control cells. Long term genistein-treated cells retained their responsiveness to genistein’s anti-motility effect. Conclusions Genistein inhibits pathways in human prostate that drive transformation to a lethal high motility phenotype. Long term treatment induces compensatory changes in biomarkers of efficacy. The current strategy of using such biomarkers after short term intervention as go/ no-go determinants in early phase chemoprevention trials should be carefully examined.

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