Genic microsatellite markers derived from EST sequences of switchgrass (Panicum virgatum L.)

Christian M. Tobias, Daniel M. Hayden, Paul Twigg, Gautam Sarath

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Switchgrass is a large, North American, perennial grass that is being evaluated as a potential energy crop. There is a need to assess genetic diversity in stored accessions and in remaining native stands to assist breeding and conservation efforts. Marker development will also be necessary for genetic linkage mapping. Toward this end, 32 switchgrass genic di-, tri- and tetranucleotide repeat microsatellites were identified from expressed sequence tags (ESTs). These microsatellites were used to screen individuals from two different named cultivars. The markers displayed a high level of polymorphism consistent with the tetraploid, allogamous behaviour of the cultivars tested.

Original languageEnglish (US)
Pages (from-to)185-187
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
StatePublished - Mar 1 2006

Fingerprint

Panicum
Panicum virgatum
Expressed Sequence Tags
expressed sequence tags
Microsatellite Repeats
cultivar
microsatellite repeats
energy crop
potential energy
Trinucleotide Repeats
polymorphism
Genetic Linkage
Tetraploidy
Chromosome Mapping
breeding
grass
Poaceae
Potential energy
Polymorphism
Crops

Keywords

  • Energy crop
  • Genetic diversity
  • Polyploid
  • Simple sequence repeat

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Genic microsatellite markers derived from EST sequences of switchgrass (Panicum virgatum L.). / Tobias, Christian M.; Hayden, Daniel M.; Twigg, Paul; Sarath, Gautam.

In: Molecular Ecology Notes, Vol. 6, No. 1, 01.03.2006, p. 185-187.

Research output: Contribution to journalArticle

Tobias, Christian M. ; Hayden, Daniel M. ; Twigg, Paul ; Sarath, Gautam. / Genic microsatellite markers derived from EST sequences of switchgrass (Panicum virgatum L.). In: Molecular Ecology Notes. 2006 ; Vol. 6, No. 1. pp. 185-187.
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