Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model

Peter C.H. Feng, Steven R. Monday, David W. Lacher, Lesley Allison, Anja Siitonen, Christine Keys, Marjut Eklund, Hideki Nagano, Helge Karch, James Keen, Thomas S. Whittam

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of β-glucuronidase-positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal strain and shared sequence type (ST)-65 by multilocus sequence typing (MLST); thus, they belong in A5. However, these strains exhibited pulsed-field gel electrophoresis (PFGE) profile differences that suggested genomic divergence between populations. Sorbitol-fermenting O157 (SFO157) strains from Finland, Scotland, and Germany were identical to A4 clonal strain and belong in A4. Some SFO157 strains, isolated years apart and from different countries, had identical PFGE profiles, suggesting a common origin. Despite similarities, some Finnish and Scottish and all of the German strains have ST-75 ("German clone"), whereas others have ST-76, a new variant ("Scottish clone""). MLST of strains in other clonal complexes also discriminated strains thought to be identical and showed that genetic differences will further distinguish clonal populations into subclones.

Original languageEnglish (US)
Pages (from-to)1701-1706
Number of pages6
JournalEmerging infectious diseases
Volume13
Issue number11
DOIs
StatePublished - Jan 1 2007

Fingerprint

Escherichia coli O157
Sorbitol
Multilocus Sequence Typing
Pulsed Field Gel Electrophoresis
Clone Cells
Glucuronidase
Scotland
Finland
Population
Germany
Japan
Mutation

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Feng, P. C. H., Monday, S. R., Lacher, D. W., Allison, L., Siitonen, A., Keys, C., ... Whittam, T. S. (2007). Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model. Emerging infectious diseases, 13(11), 1701-1706. https://doi.org/10.3201/eid1311.070381

Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model. / Feng, Peter C.H.; Monday, Steven R.; Lacher, David W.; Allison, Lesley; Siitonen, Anja; Keys, Christine; Eklund, Marjut; Nagano, Hideki; Karch, Helge; Keen, James; Whittam, Thomas S.

In: Emerging infectious diseases, Vol. 13, No. 11, 01.01.2007, p. 1701-1706.

Research output: Contribution to journalArticle

Feng, PCH, Monday, SR, Lacher, DW, Allison, L, Siitonen, A, Keys, C, Eklund, M, Nagano, H, Karch, H, Keen, J & Whittam, TS 2007, 'Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model', Emerging infectious diseases, vol. 13, no. 11, pp. 1701-1706. https://doi.org/10.3201/eid1311.070381
Feng, Peter C.H. ; Monday, Steven R. ; Lacher, David W. ; Allison, Lesley ; Siitonen, Anja ; Keys, Christine ; Eklund, Marjut ; Nagano, Hideki ; Karch, Helge ; Keen, James ; Whittam, Thomas S. / Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model. In: Emerging infectious diseases. 2007 ; Vol. 13, No. 11. pp. 1701-1706.
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