Genetic differences in hemoglobin function between highland and lowland deer mice

Jay F Storz, Amy M. Runck, Hideaki Moriyama, Roy E. Weber, Angela Fago

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

In high-altitude vertebrates, adaptive changes in blood-O2 affinity may be mediated by modifications of hemoglobin (Hb) structure that affect intrinsic O2 affinity and/or responsiveness to allosteric effectors that modulate Hb-O2 affinity. This mode of genotypic specialization is considered typical of mammalian species that are high-altitude natives. Here we investigated genetically based differences in Hb-O2 affinity between highland and lowland populations of the deer mouse (Peromyscus maniculatus), a generalist species that has the broadest altitudinal distribution of any North American mammal. The results of a combined genetic and proteomic analysis revealed that deer mice harbor a high level of Hb isoform diversity that is attributable to allelic polymorphism at two tandemly duplicated α-globin genes and two tandemly duplicated β-globin genes. This high level of isoHb diversity translates into a correspondingly high level of interindividual variation in Hb functional properties. O2 equilibrium experiments revealed that the Hbs of highland mice exhibit slightly higher intrinsic O2 affinities and significantly lower Cl- sensitivities relative to the Hbs of lowland mice. The experiments also revealed distinct biochemical properties of deer mouse Hb related to the anion-dependent allosteric regulation of O2 affinity. In conjunction with previous findings, our results demonstrate that modifications of Hb structure that alter allosteric anion sensitivity play an important role in the adaptive fine-tuning of blood-O2 affinity.

Original languageEnglish (US)
Pages (from-to)2565-2574
Number of pages10
JournalJournal of Experimental Biology
Volume213
Issue number15
DOIs
StatePublished - Aug 1 2010

Fingerprint

Peromyscus
hemoglobin
deer
lowlands
Hemoglobins
highlands
Globins
anions
Anions
anion
blood
Allosteric Regulation
Peromyscus maniculatus
proteomics
gene
mice
Population Groups
generalist
Proteomics
Genes

Keywords

  • High-altitude adaptation
  • Hypoxia
  • Oxygen transport
  • Peromyscus
  • Physiological genetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Cite this

Genetic differences in hemoglobin function between highland and lowland deer mice. / Storz, Jay F; Runck, Amy M.; Moriyama, Hideaki; Weber, Roy E.; Fago, Angela.

In: Journal of Experimental Biology, Vol. 213, No. 15, 01.08.2010, p. 2565-2574.

Research output: Contribution to journalArticle

Storz, Jay F ; Runck, Amy M. ; Moriyama, Hideaki ; Weber, Roy E. ; Fago, Angela. / Genetic differences in hemoglobin function between highland and lowland deer mice. In: Journal of Experimental Biology. 2010 ; Vol. 213, No. 15. pp. 2565-2574.
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