Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae

E. I. Stepchenkova, S. G. Kozmin, V. V. Alenin, Youri I Pavlov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We studied the effect of inactivation of genes, which control biosynthesis of inosine monophosphate (IMP) de novo and purine salvage and interconversion pathways, on sensitivity of yeast Saccharomyces cerevisiae to the mutagenic and toxic action of 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA). It was shown that the manifestation of HAP and AHA mutagenic properties depends on the action of enzyme adenine phosphoribosyltransferase encoded in yeast by APT1 gene. A blockade of any step of IMP biosynthesis, with the exception of the block mediated by inactivation of genes ADE16 and ADE17 leading to the accumulation of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), was shown to enhance yeast cell sensitivity to the HAP mutagenic effect; however, it does not affect the sensitivity to AHA. A block of conversion of IMP into adenosine monophosphate (AMP) causes hypersensitivity of yeast cells to the mutagenic action of HAP and to the toxic effect of HAP, AHA, and hypoxanthine. It is possible that this enhancement of sensitivity to HAP and AHA is due to changes in the pool of purines. We conclude that genes ADE12, ADE13, AAH1, and HAM1 controlling processes of purine salvage and interconversion in yeast, make the greatest contribution to the protection against the toxic and mutagenic action of the examined analogs. Possible mechanisms of HAP detoxication in bacteria, yeast, and humans are discussed.

Original languageEnglish (US)
Pages (from-to)409-414
Number of pages6
JournalRussian Journal of Genetics
Volume45
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Saccharomyces cerevisiae
Yeasts
Inosine Monophosphate
Toxic Actions
Gene Silencing
Adenine Phosphoribosyltransferase
Purines
Hypoxanthine
Poisons
Adenosine Monophosphate
Genes
purine
Hypersensitivity
Bacteria
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae. / Stepchenkova, E. I.; Kozmin, S. G.; Alenin, V. V.; Pavlov, Youri I.

In: Russian Journal of Genetics, Vol. 45, No. 4, 01.04.2009, p. 409-414.

Research output: Contribution to journalArticle

@article{9b31b314ada54098bbabd8d1e055a20d,
title = "Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae",
abstract = "We studied the effect of inactivation of genes, which control biosynthesis of inosine monophosphate (IMP) de novo and purine salvage and interconversion pathways, on sensitivity of yeast Saccharomyces cerevisiae to the mutagenic and toxic action of 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA). It was shown that the manifestation of HAP and AHA mutagenic properties depends on the action of enzyme adenine phosphoribosyltransferase encoded in yeast by APT1 gene. A blockade of any step of IMP biosynthesis, with the exception of the block mediated by inactivation of genes ADE16 and ADE17 leading to the accumulation of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), was shown to enhance yeast cell sensitivity to the HAP mutagenic effect; however, it does not affect the sensitivity to AHA. A block of conversion of IMP into adenosine monophosphate (AMP) causes hypersensitivity of yeast cells to the mutagenic action of HAP and to the toxic effect of HAP, AHA, and hypoxanthine. It is possible that this enhancement of sensitivity to HAP and AHA is due to changes in the pool of purines. We conclude that genes ADE12, ADE13, AAH1, and HAM1 controlling processes of purine salvage and interconversion in yeast, make the greatest contribution to the protection against the toxic and mutagenic action of the examined analogs. Possible mechanisms of HAP detoxication in bacteria, yeast, and humans are discussed.",
author = "Stepchenkova, {E. I.} and Kozmin, {S. G.} and Alenin, {V. V.} and Pavlov, {Youri I}",
year = "2009",
month = "4",
day = "1",
doi = "10.1134/S1022795409040048",
language = "English (US)",
volume = "45",
pages = "409--414",
journal = "Russian Journal of Genetics",
issn = "1022-7954",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "4",

}

TY - JOUR

T1 - Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae

AU - Stepchenkova, E. I.

AU - Kozmin, S. G.

AU - Alenin, V. V.

AU - Pavlov, Youri I

PY - 2009/4/1

Y1 - 2009/4/1

N2 - We studied the effect of inactivation of genes, which control biosynthesis of inosine monophosphate (IMP) de novo and purine salvage and interconversion pathways, on sensitivity of yeast Saccharomyces cerevisiae to the mutagenic and toxic action of 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA). It was shown that the manifestation of HAP and AHA mutagenic properties depends on the action of enzyme adenine phosphoribosyltransferase encoded in yeast by APT1 gene. A blockade of any step of IMP biosynthesis, with the exception of the block mediated by inactivation of genes ADE16 and ADE17 leading to the accumulation of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), was shown to enhance yeast cell sensitivity to the HAP mutagenic effect; however, it does not affect the sensitivity to AHA. A block of conversion of IMP into adenosine monophosphate (AMP) causes hypersensitivity of yeast cells to the mutagenic action of HAP and to the toxic effect of HAP, AHA, and hypoxanthine. It is possible that this enhancement of sensitivity to HAP and AHA is due to changes in the pool of purines. We conclude that genes ADE12, ADE13, AAH1, and HAM1 controlling processes of purine salvage and interconversion in yeast, make the greatest contribution to the protection against the toxic and mutagenic action of the examined analogs. Possible mechanisms of HAP detoxication in bacteria, yeast, and humans are discussed.

AB - We studied the effect of inactivation of genes, which control biosynthesis of inosine monophosphate (IMP) de novo and purine salvage and interconversion pathways, on sensitivity of yeast Saccharomyces cerevisiae to the mutagenic and toxic action of 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA). It was shown that the manifestation of HAP and AHA mutagenic properties depends on the action of enzyme adenine phosphoribosyltransferase encoded in yeast by APT1 gene. A blockade of any step of IMP biosynthesis, with the exception of the block mediated by inactivation of genes ADE16 and ADE17 leading to the accumulation of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), was shown to enhance yeast cell sensitivity to the HAP mutagenic effect; however, it does not affect the sensitivity to AHA. A block of conversion of IMP into adenosine monophosphate (AMP) causes hypersensitivity of yeast cells to the mutagenic action of HAP and to the toxic effect of HAP, AHA, and hypoxanthine. It is possible that this enhancement of sensitivity to HAP and AHA is due to changes in the pool of purines. We conclude that genes ADE12, ADE13, AAH1, and HAM1 controlling processes of purine salvage and interconversion in yeast, make the greatest contribution to the protection against the toxic and mutagenic action of the examined analogs. Possible mechanisms of HAP detoxication in bacteria, yeast, and humans are discussed.

UR - http://www.scopus.com/inward/record.url?scp=65349099312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65349099312&partnerID=8YFLogxK

U2 - 10.1134/S1022795409040048

DO - 10.1134/S1022795409040048

M3 - Article

AN - SCOPUS:65349099312

VL - 45

SP - 409

EP - 414

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 4

ER -