Generation of Functional Human Retinal Ganglion Cells with Target Specificity from Pluripotent Stem Cells by Chemically Defined Recapitulation of Developmental Mechanism

Pooja Teotia, Divyan A. Chopra, Shashank Manohar Dravid, Matthew Van Hook, Fang Qiu, John Morrison, A Angie Rizzino, Iqbal Ahmad

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glaucoma is a complex group of diseases wherein a selective degeneration of retinal ganglion cells (RGCs) lead to irreversible loss of vision. A comprehensive approach to glaucomatous RGC degeneration may include stem cells to functionally replace dead neurons through transplantation and understand RGCs vulnerability using a disease in a dish stem cell model. Both approaches require the directed generation of stable, functional, and target-specific RGCs from renewable sources of cells, that is, the embryonic stem cells and induced pluripotent stem cells. Here, we demonstrate a rapid and safe, stage-specific, chemically defined protocol that selectively generates RGCs across species, including human, by recapitulating the developmental mechanism. The de novo generated RGCs from pluripotent cells are similar to native RGCs at the molecular, biochemical, functional levels. They also express axon guidance molecules, and discriminate between specific and nonspecific targets, and are nontumorigenic. Stem Cells 2017;35:572–585.

Original languageEnglish (US)
Pages (from-to)572-585
Number of pages14
JournalSTEM CELLS
Volume35
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Pluripotent Stem Cells
Retinal Ganglion Cells
Stem Cells
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Glaucoma
Transplantation
Neurons

Keywords

  • Chemically defined medium
  • Directed differentiation
  • Embryonic stem cells
  • Glaucoma
  • Induced pluripotent stem cells
  • Retinal ganglion cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Generation of Functional Human Retinal Ganglion Cells with Target Specificity from Pluripotent Stem Cells by Chemically Defined Recapitulation of Developmental Mechanism. / Teotia, Pooja; Chopra, Divyan A.; Dravid, Shashank Manohar; Van Hook, Matthew; Qiu, Fang; Morrison, John; Rizzino, A Angie; Ahmad, Iqbal.

In: STEM CELLS, Vol. 35, No. 3, 01.03.2017, p. 572-585.

Research output: Contribution to journalArticle

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