Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking

Ruthellen H. Anderson, Jason G. Kerkvliet, Jaelin J. Otta, Alan D. Ross, Patricia C. Leiferman, Adam D. Hoppe, Kevin R. Francis

Research output: Contribution to journalArticle

Abstract

The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalStem Cell Research
Volume33
DOIs
StatePublished - Dec 2018

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ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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