Gene transfer via nucleofection into adult and embryonic stem cells.

Uma Lakshmipathy, Shannon Buckley, Catherine Verfaillie

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The use of embryonic and adult stem cells as therapeutic agents is gaining momentum. A major impediment in the use of stem cells for genetic disorders is their ability to undergo genetic modification. The recognition of various site-specific integration methods open up a new avenue for gene therapy in stem cells. However, this necessitates efficient delivery of DNA molecule into cells. Most commercially used liposome-mediated transfection reagents are toxic or work poorly with stem cells. Electroporation, while effective in transfecting stem cells, is rather harsh and leads to excessive cell death. Nucleofection, a technology by Amaxa, uses a combination of electric pulse in an appropriate media, which decreases the toxicity and promotes efficient transfection of stem cells. Various types of adult and embryonic stem cells can be successfully transfected using this method, as described in this chapter.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume407
StatePublished - 2007

Fingerprint

Adult Stem Cells
Embryonic Stem Cells
Stem Cells
Genes
Transfection
Inborn Genetic Diseases
Electroporation
Poisons
Liposomes
Genetic Therapy
Cell Death
Technology
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gene transfer via nucleofection into adult and embryonic stem cells. / Lakshmipathy, Uma; Buckley, Shannon; Verfaillie, Catherine.

In: Methods in molecular biology (Clifton, N.J.), Vol. 407, 2007, p. 115-126.

Research output: Contribution to journalArticle

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