Gene expression profiling of tolerant barley in response to diuraphis noxia (Hemiptera: Aphididae) feeding

A. Gutsche, T. Heng-Moss, G. Sarath, Paul Twigg, Y. Xia, Guoqing Lu, D. Mornhinweg

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Aphids are, arguably, the single most damaging group of agricultural insect pests throughout the world. Plant tolerance, which is a plant response to an insect pest, is viewed as an excellent management strategy. Developing testable hypotheses based on genome-wide and more focused methods will help in understanding the molecular underpinnings of plant tolerance to aphid herbivory. As a first step in this process, we undertook transcript profiling with Affymetrix GeneChip Barley Genome arrays using RNA extracted from tissues of tolerant and susceptible genotypes collected at three hours, three days and six days after Diuraphis noxia introduction. Acquired data were compared to identify changes unique to the tolerant barley at each harvest date. Transcript abundance of 4086 genes was differentially changed over the three harvest dates in tolerant and susceptible barley in response to D. noxia feeding. Across the three harvest dates, the greatest number of genes was differentially expressed in both barleys at three days after aphid introduction. A total of 909 genes showed significant levels of change in the tolerant barley in response to D. noxia feeding as compared to susceptible plants infested with aphids. Many of these genes could be assigned to specific metabolic categories, including several associated with plant defense and scavenging of reactive oxygen species (ROS). Interestingly, two peroxidase genes, designated HvPRXA1 and HvPRXA2, were up-regulated to a greater degree in response to D. noxia feeding on tolerant barley plants, indicating that specific peroxidases could be important for the tolerance process. These findings suggest that the ability to elevate and sustain levels of ROS-scavenging enzymes could play an important role in the tolerant response.

Original languageEnglish (US)
Pages (from-to)163-173
Number of pages11
JournalBulletin of Entomological Research
Volume99
Issue number2
DOIs
StatePublished - Apr 1 2009

Fingerprint

Diuraphis noxia
Aphididae
Hemiptera
barley
gene expression
Aphidoidea
harvest date
genes
insect pests
reactive oxygen species
genome
peroxidases
plant response
peroxidase
herbivores
RNA
genotype
enzymes

Keywords

  • Microarray analysis
  • Peroxidases
  • Plant resistance
  • Russian wheat aphid

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Gene expression profiling of tolerant barley in response to diuraphis noxia (Hemiptera : Aphididae) feeding. / Gutsche, A.; Heng-Moss, T.; Sarath, G.; Twigg, Paul; Xia, Y.; Lu, Guoqing; Mornhinweg, D.

In: Bulletin of Entomological Research, Vol. 99, No. 2, 01.04.2009, p. 163-173.

Research output: Contribution to journalArticle

Gutsche, A. ; Heng-Moss, T. ; Sarath, G. ; Twigg, Paul ; Xia, Y. ; Lu, Guoqing ; Mornhinweg, D. / Gene expression profiling of tolerant barley in response to diuraphis noxia (Hemiptera : Aphididae) feeding. In: Bulletin of Entomological Research. 2009 ; Vol. 99, No. 2. pp. 163-173.
@article{4349a424f1ad4ef0b964a12f723c7957,
title = "Gene expression profiling of tolerant barley in response to diuraphis noxia (Hemiptera: Aphididae) feeding",
abstract = "Aphids are, arguably, the single most damaging group of agricultural insect pests throughout the world. Plant tolerance, which is a plant response to an insect pest, is viewed as an excellent management strategy. Developing testable hypotheses based on genome-wide and more focused methods will help in understanding the molecular underpinnings of plant tolerance to aphid herbivory. As a first step in this process, we undertook transcript profiling with Affymetrix GeneChip Barley Genome arrays using RNA extracted from tissues of tolerant and susceptible genotypes collected at three hours, three days and six days after Diuraphis noxia introduction. Acquired data were compared to identify changes unique to the tolerant barley at each harvest date. Transcript abundance of 4086 genes was differentially changed over the three harvest dates in tolerant and susceptible barley in response to D. noxia feeding. Across the three harvest dates, the greatest number of genes was differentially expressed in both barleys at three days after aphid introduction. A total of 909 genes showed significant levels of change in the tolerant barley in response to D. noxia feeding as compared to susceptible plants infested with aphids. Many of these genes could be assigned to specific metabolic categories, including several associated with plant defense and scavenging of reactive oxygen species (ROS). Interestingly, two peroxidase genes, designated HvPRXA1 and HvPRXA2, were up-regulated to a greater degree in response to D. noxia feeding on tolerant barley plants, indicating that specific peroxidases could be important for the tolerance process. These findings suggest that the ability to elevate and sustain levels of ROS-scavenging enzymes could play an important role in the tolerant response.",
keywords = "Microarray analysis, Peroxidases, Plant resistance, Russian wheat aphid",
author = "A. Gutsche and T. Heng-Moss and G. Sarath and Paul Twigg and Y. Xia and Guoqing Lu and D. Mornhinweg",
year = "2009",
month = "4",
day = "1",
doi = "10.1017/S0007485308006184",
language = "English (US)",
volume = "99",
pages = "163--173",
journal = "Bulletin of Entomological Research",
issn = "0007-4853",
publisher = "Cambridge University Press",
number = "2",

}

TY - JOUR

T1 - Gene expression profiling of tolerant barley in response to diuraphis noxia (Hemiptera

T2 - Aphididae) feeding

AU - Gutsche, A.

AU - Heng-Moss, T.

AU - Sarath, G.

AU - Twigg, Paul

AU - Xia, Y.

AU - Lu, Guoqing

AU - Mornhinweg, D.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Aphids are, arguably, the single most damaging group of agricultural insect pests throughout the world. Plant tolerance, which is a plant response to an insect pest, is viewed as an excellent management strategy. Developing testable hypotheses based on genome-wide and more focused methods will help in understanding the molecular underpinnings of plant tolerance to aphid herbivory. As a first step in this process, we undertook transcript profiling with Affymetrix GeneChip Barley Genome arrays using RNA extracted from tissues of tolerant and susceptible genotypes collected at three hours, three days and six days after Diuraphis noxia introduction. Acquired data were compared to identify changes unique to the tolerant barley at each harvest date. Transcript abundance of 4086 genes was differentially changed over the three harvest dates in tolerant and susceptible barley in response to D. noxia feeding. Across the three harvest dates, the greatest number of genes was differentially expressed in both barleys at three days after aphid introduction. A total of 909 genes showed significant levels of change in the tolerant barley in response to D. noxia feeding as compared to susceptible plants infested with aphids. Many of these genes could be assigned to specific metabolic categories, including several associated with plant defense and scavenging of reactive oxygen species (ROS). Interestingly, two peroxidase genes, designated HvPRXA1 and HvPRXA2, were up-regulated to a greater degree in response to D. noxia feeding on tolerant barley plants, indicating that specific peroxidases could be important for the tolerance process. These findings suggest that the ability to elevate and sustain levels of ROS-scavenging enzymes could play an important role in the tolerant response.

AB - Aphids are, arguably, the single most damaging group of agricultural insect pests throughout the world. Plant tolerance, which is a plant response to an insect pest, is viewed as an excellent management strategy. Developing testable hypotheses based on genome-wide and more focused methods will help in understanding the molecular underpinnings of plant tolerance to aphid herbivory. As a first step in this process, we undertook transcript profiling with Affymetrix GeneChip Barley Genome arrays using RNA extracted from tissues of tolerant and susceptible genotypes collected at three hours, three days and six days after Diuraphis noxia introduction. Acquired data were compared to identify changes unique to the tolerant barley at each harvest date. Transcript abundance of 4086 genes was differentially changed over the three harvest dates in tolerant and susceptible barley in response to D. noxia feeding. Across the three harvest dates, the greatest number of genes was differentially expressed in both barleys at three days after aphid introduction. A total of 909 genes showed significant levels of change in the tolerant barley in response to D. noxia feeding as compared to susceptible plants infested with aphids. Many of these genes could be assigned to specific metabolic categories, including several associated with plant defense and scavenging of reactive oxygen species (ROS). Interestingly, two peroxidase genes, designated HvPRXA1 and HvPRXA2, were up-regulated to a greater degree in response to D. noxia feeding on tolerant barley plants, indicating that specific peroxidases could be important for the tolerance process. These findings suggest that the ability to elevate and sustain levels of ROS-scavenging enzymes could play an important role in the tolerant response.

KW - Microarray analysis

KW - Peroxidases

KW - Plant resistance

KW - Russian wheat aphid

UR - http://www.scopus.com/inward/record.url?scp=65549159564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549159564&partnerID=8YFLogxK

U2 - 10.1017/S0007485308006184

DO - 10.1017/S0007485308006184

M3 - Article

C2 - 18840314

AN - SCOPUS:65549159564

VL - 99

SP - 163

EP - 173

JO - Bulletin of Entomological Research

JF - Bulletin of Entomological Research

SN - 0007-4853

IS - 2

ER -