Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Sarah M. Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A. Talbott, Robert A. Cushman, Jennifer R Wood, John S Davis, Andrea S Cupp

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into cell-specific behaviors and the differentiation process that transforms somatic follicular cells into luteal cells.

Original languageEnglish (US)
Pages (from-to)379-394
Number of pages16
JournalMolecular and Cellular Endocrinology
Volume439
DOIs
StatePublished - Jan 5 2017

Fingerprint

Luteal Cells
Gene Expression Profiling
Gene expression
Genes
Microarrays
Ontology
Cells
RNA
Enzymes
Theca Cells
Molecular Sequence Annotation
Gene Ontology
Ovarian Follicle
Granulosa Cells
Corpus Luteum
Cell Lineage
Ovulation
Genetic Markers
Transcriptome
Gene Expression

Keywords

  • Bovine
  • Cell identity
  • Corpus luteum
  • Follicle
  • Ovary
  • Transcriptome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions. / Romereim, Sarah M.; Summers, Adam F.; Pohlmeier, William E.; Zhang, Pan; Hou, Xiaoying; Talbott, Heather A.; Cushman, Robert A.; Wood, Jennifer R; Davis, John S; Cupp, Andrea S.

In: Molecular and Cellular Endocrinology, Vol. 439, 05.01.2017, p. 379-394.

Research output: Contribution to journalArticle

Romereim, Sarah M. ; Summers, Adam F. ; Pohlmeier, William E. ; Zhang, Pan ; Hou, Xiaoying ; Talbott, Heather A. ; Cushman, Robert A. ; Wood, Jennifer R ; Davis, John S ; Cupp, Andrea S. / Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions. In: Molecular and Cellular Endocrinology. 2017 ; Vol. 439. pp. 379-394.
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