Gene Expression Following Direct Injection of DNA into Liver

M. Anne Hickman, Robert W. Malone, Tracey R. Sih, Daren Knoell, Karin Lehmann-Bruinsma, Francis C. Szoka, Rosemary Walzem, Don M. Carlson, Jerry S. Powell

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

The liver is an attractive target tissue for gene therapy. Current approaches for hepatic gene delivery include retroviral and adenoviral vectors, liposome/DNA, and peptide/DNA complexes. This study describes a technique for direct injection of DNA into liver that led to significant gene expression. Gene expression was characterized in both rats and cats following injection of plasmid DNA encoding several different proteins. Luciferase activity was measured after injection of plasmid DNA encoding the luciferase gene (pCMVL), β-galactosidase (β-Gal) activity was evaluated in situ using plasmid DNA encoding Lac Z (pCMVβ), and serum concentration of secreted human α-1-antitrypsin was measured following injection of plasmid DNA encoding this protein (pRC/CMV-sHAT). Several variables, including injection technique, DNA dose, and DNA diluent, were investigated. Direct injection of pCMVL resulted in maximal luciferase expression at 24–48 hr. β-Gal staining demonstrated that the majority of transfected hepatocytes were located near the injection site. Significant concentrations of human α-1-antitrypsin were detected in the serum of animals injected with pRC/CMV-sHAT. These findings demonstrate the general principle that direct injection of plasmid DNA into liver can lead to significant gene expression.

Original languageEnglish (US)
Pages (from-to)1477-1483
Number of pages7
JournalHuman gene therapy
Volume5
Issue number12
DOIs
StatePublished - Dec 1 1994

Fingerprint

Gene Expression
Injections
Liver
DNA
Plasmids
Luciferases
Galactosidases
Cell- and Tissue-Based Therapy
Serum
Liposomes
Genetic Therapy
Genes
Hepatocytes
Proteins
Cats
Staining and Labeling
Peptides

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Hickman, M. A., Malone, R. W., Sih, T. R., Knoell, D., Lehmann-Bruinsma, K., Szoka, F. C., ... Powell, J. S. (1994). Gene Expression Following Direct Injection of DNA into Liver. Human gene therapy, 5(12), 1477-1483. https://doi.org/10.1089/hum.1994.5.12-1477

Gene Expression Following Direct Injection of DNA into Liver. / Hickman, M. Anne; Malone, Robert W.; Sih, Tracey R.; Knoell, Daren; Lehmann-Bruinsma, Karin; Szoka, Francis C.; Walzem, Rosemary; Carlson, Don M.; Powell, Jerry S.

In: Human gene therapy, Vol. 5, No. 12, 01.12.1994, p. 1477-1483.

Research output: Contribution to journalArticle

Hickman, MA, Malone, RW, Sih, TR, Knoell, D, Lehmann-Bruinsma, K, Szoka, FC, Walzem, R, Carlson, DM & Powell, JS 1994, 'Gene Expression Following Direct Injection of DNA into Liver', Human gene therapy, vol. 5, no. 12, pp. 1477-1483. https://doi.org/10.1089/hum.1994.5.12-1477
Hickman MA, Malone RW, Sih TR, Knoell D, Lehmann-Bruinsma K, Szoka FC et al. Gene Expression Following Direct Injection of DNA into Liver. Human gene therapy. 1994 Dec 1;5(12):1477-1483. https://doi.org/10.1089/hum.1994.5.12-1477
Hickman, M. Anne ; Malone, Robert W. ; Sih, Tracey R. ; Knoell, Daren ; Lehmann-Bruinsma, Karin ; Szoka, Francis C. ; Walzem, Rosemary ; Carlson, Don M. ; Powell, Jerry S. / Gene Expression Following Direct Injection of DNA into Liver. In: Human gene therapy. 1994 ; Vol. 5, No. 12. pp. 1477-1483.
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