Gel electrophoresis of partially denatured DNA. Retardation effect

Its analysis and application

V. I. Lyamichev, I. G. Panyutin, Yuri L Lyubchenko

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the melted regions by a prooedure allowing the denatured state to be fixed at any point within the melting range. The approach and the availability of the melting maps of DNAs made it possible to investigate DNA molecules differing in length and in the size of the melted regions. The presence of a denatured region at the end of the molecule or inside of it was shown to decrease its electrophoretio mobility, the effect depending on the size of the melted region and on the DNA length. On the basis of the experimental results an explanation is proposed for the cause of retardation in the case of partially denatured DNA.

Original languageEnglish (US)
Pages (from-to)4813-4826
Number of pages14
JournalNucleic acids research
Volume10
Issue number15
DOIs
StatePublished - Aug 11 1982

Fingerprint

Electrophoresis
DNA
Gels
Molecules
Melting
Glyoxal
Nucleic Acid Denaturation
Freezing
Availability
Partial
Decrease
Experimental Results
Range of data

ASJC Scopus subject areas

  • Genetics

Cite this

Gel electrophoresis of partially denatured DNA. Retardation effect : Its analysis and application. / Lyamichev, V. I.; Panyutin, I. G.; Lyubchenko, Yuri L.

In: Nucleic acids research, Vol. 10, No. 15, 11.08.1982, p. 4813-4826.

Research output: Contribution to journalArticle

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