Gamma radiation inhibits fibroblast-mediated collagen gel retraction

Stefano Carnevali, T. Mio, Y. Adachi, J. R. Spurzem, I. Striz, Debra Romberger, M. Illig, S. I. Rennard

Research output: Contribution to journalArticle

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Abstract

Radiation exposure is known to impair healing in irradiated areas. Fibroblasts play a major role in the production and modification of extracellular matrix in wound repair. Since one important aspect of wound repair is the contraction of the wound, this study investigated the effects of radiation on the ability of fibroblasts to mediate collagen gel contraction in an in vitro model of wound retraction. After irradiation, the cells were detached and suspended in a solution of rat tail tendon collagen. Radiation exposure decreased retraction, and this effect was dose dependent. In order to define the mechanism of reduced gel retraction, we investigated α2β1 cell surface integrin and fibronectin, which are thought to mediate contraction, and prostaglandin E2 (PGE2), which is known to inhibit this process. PGE2 release increased dose responsively following radiation. The cyclooxygenase inhibitor indomethacin could partially restore the contractile activity of irradiated fibroblasts. Fibronectin production in gel culture showed a significant decrease. In contrast, there was no decrease in α2β1 integrin expression in radiated cells. In conclusion, radiation decreases fibroblast-mediated gel contraction. Increased PGE2 production and decreased fibronectin production by irradiated fibroblasts may contribute to this effect and may be in part responsible for poor heading of radiated tissue.

Original languageEnglish (US)
Pages (from-to)459-469
Number of pages11
JournalTissue and Cell
Volume35
Issue number6
DOIs
StatePublished - Dec 2003

Fingerprint

Gamma Rays
gamma radiation
fibroblasts
collagen
Collagen
Fibroblasts
animal injuries
Gels
gels
fibronectins
Fibronectins
Dinoprostone
prostaglandins
integrins
Wounds and Injuries
Integrins
Radiation
Cyclooxygenase Inhibitors
indomethacin
Radiation Effects

Keywords

  • Contraction
  • Extracellular matrix
  • Fibroblast
  • Fibronectin
  • PGE

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Carnevali, S., Mio, T., Adachi, Y., Spurzem, J. R., Striz, I., Romberger, D., ... Rennard, S. I. (2003). Gamma radiation inhibits fibroblast-mediated collagen gel retraction. Tissue and Cell, 35(6), 459-469. https://doi.org/10.1016/S0040-8166(03)00072-7

Gamma radiation inhibits fibroblast-mediated collagen gel retraction. / Carnevali, Stefano; Mio, T.; Adachi, Y.; Spurzem, J. R.; Striz, I.; Romberger, Debra; Illig, M.; Rennard, S. I.

In: Tissue and Cell, Vol. 35, No. 6, 12.2003, p. 459-469.

Research output: Contribution to journalArticle

Carnevali, S, Mio, T, Adachi, Y, Spurzem, JR, Striz, I, Romberger, D, Illig, M & Rennard, SI 2003, 'Gamma radiation inhibits fibroblast-mediated collagen gel retraction', Tissue and Cell, vol. 35, no. 6, pp. 459-469. https://doi.org/10.1016/S0040-8166(03)00072-7
Carnevali, Stefano ; Mio, T. ; Adachi, Y. ; Spurzem, J. R. ; Striz, I. ; Romberger, Debra ; Illig, M. ; Rennard, S. I. / Gamma radiation inhibits fibroblast-mediated collagen gel retraction. In: Tissue and Cell. 2003 ; Vol. 35, No. 6. pp. 459-469.
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