Galactosyl transferase activity in rat bladder transitional cell carcinoma lines and in exfoliated cells in urine during carcinogenesis and reversible hyperplasia

G. M. Plotkin, S. L. Gilbert, R. J. Wides, G. Wolf, S. M. Cohen, S. Fukushima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cultured cells of rat bladder transitional cell carcinoma line AY-27, in suspension, were assayed for glactosyl transferase (GT) by measurement of the transfer of [ 3H]galactose from uridine diphosphate-[ 3H]galactose to desialylated ovine submaxillary mucin (OSM-NANA). The assay was optimized with respect to time and to protein, uridine diphosphate galactose, OSM-NANA and Triton X-100 concentrations. This assay was then applied weekly to suspensions of exfoliated bladder cells collected from urines of rats fed the bladder carcinogen N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide, and of control rats. Increases in activity over controls appeared 42 weeks after feeding the carcinogen, at a stage when bladder tumors were already microinvasive or deeply invasive, and activities at 52 weeks were about 10-fold greater than normal values. In contrast, a bladder cytotoxic agent inducing reversible hyperplasia was injected into rats, and exfoliated cells were collected from urines: these cells showed no greater GT activity than normal. Bladder tumor tissue from a transplanted tumor had the same high specific enzymatic GT activity as exfoliated cells from tumor-bearing rats.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalCancer Biochemistry Biophysics
Volume4
Issue number4
StatePublished - Dec 1 1980

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Transitional Cell Carcinoma
Transferases
Hyperplasia
Carcinogenesis
Urinary Bladder
Urine
Cell Line
Uridine Diphosphate Galactose
Urinary Bladder Neoplasms
Carcinogens
Suspensions
Cytotoxins
Octoxynol
Galactose
Cultured Cells
Neoplasms
Reference Values
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Cancer Research

Cite this

Galactosyl transferase activity in rat bladder transitional cell carcinoma lines and in exfoliated cells in urine during carcinogenesis and reversible hyperplasia. / Plotkin, G. M.; Gilbert, S. L.; Wides, R. J.; Wolf, G.; Cohen, S. M.; Fukushima, S.

In: Cancer Biochemistry Biophysics, Vol. 4, No. 4, 01.12.1980, p. 251-256.

Research output: Contribution to journalArticle

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