Gaegurin 4, a peptide antibiotic of frog skin, forms voltage-dependent channels in planar lipid bilayers

Heejeong Kim, S. K. Han, J. B. Park, H. J. Baek, B. J. Lee, Pan Dong Ryu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Gaegurin 4 (GGN4) is a cationic peptide of 37 amino acids (MW 3748) isolated from the skin of Rana rugosa. It has shown a broad spectrum antimicrobial activity in vitro against Gram-negative and -positive bacteria, fungi and protozoa. To understand its mechanism of antimicrobial action, we examined the effect of GGN4 on the membrane conductance and the electrical properties of GGN4-induced pores in planar lipid bilayers under voltage clamp. Natural and synthetic GGN4 (0.01-1 μg/mL) increased the membrane conductance in a concentration-dependent manner, but GGN4 (1-23), an N- terminal fragment of the peptide with little antimicrobial activity, failed to increase the conductance. At symmetrical 100 mM KCl, unitary conductances of about 120 pS were frequently observed. Their current-voltage relations were linear and open state probabilities were close to 1, but longer closing events were seen more frequently at negative voltages. In addition, GGN4- induced pores were selective for cation over anion, the permeability ratio of K + to Cl - being 6: 1 in neutral and 7: 1 in acidic lipid bilayers. In conclusion, our results indicate that GGN4 forms voltage-dependent and cation-selective pores in planar lipid bilayers. The ionophoric property of GGN4 is likely to contribute to its antimicrobial activity.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal of Peptide Research
Volume53
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Lipid bilayers
Lipid Bilayers
Anura
Skin
Anti-Bacterial Agents
Peptides
Electric potential
Cations
Protozoa
Membranes
Ranidae
Peptide Fragments
gaegurin 4
Clamping devices
Gram-Positive Bacteria
Fungi
Gram-Negative Bacteria
Anions
Permeability
Bacteria

Keywords

  • Cation selective channel
  • Gaegurin 4
  • Membrane conductance
  • Peptide antibiotic
  • Planar lipid bilayer
  • Voltage dependence

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Gaegurin 4, a peptide antibiotic of frog skin, forms voltage-dependent channels in planar lipid bilayers. / Kim, Heejeong; Han, S. K.; Park, J. B.; Baek, H. J.; Lee, B. J.; Ryu, Pan Dong.

In: Journal of Peptide Research, Vol. 53, No. 1, 01.01.1999, p. 1-7.

Research output: Contribution to journalArticle

Kim, Heejeong ; Han, S. K. ; Park, J. B. ; Baek, H. J. ; Lee, B. J. ; Ryu, Pan Dong. / Gaegurin 4, a peptide antibiotic of frog skin, forms voltage-dependent channels in planar lipid bilayers. In: Journal of Peptide Research. 1999 ; Vol. 53, No. 1. pp. 1-7.
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