Fundamentals of DNA-chip/array technology for comparative gene-expression analysis

Hans Peter Saluz, Javeed Iqbal, Gino V. Limmon, Andre Ruryk, Zhihao Wu

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8 Scopus citations

Abstract

In this article we review the state-of-the-art of DNA-chip/array technology and its application in biological and medical sciences. With many of the technological hurdles to overcome array fabrication, many academic groups and numerous specialized companies are developing new tools and strategies for exploiting this promising field. DNA-microarrays are fabricated either by in situ synthesis or by conventional means, e.g. amplification of specific target DNA, followed by immobilization on the surface of various miniaturized substrates. The immobilized DNA molecules are hybridized with labelled probes. One single experiment can encompass aspects of many thousands of genes simultaneously. The unprecedented speed and parallelism of this technology has begun to have profound impacts on biological, environmental, pharmacological and medical research.

Original languageEnglish (US)
Pages (from-to)829-833
Number of pages5
JournalCurrent Science
Volume83
Issue number7
Publication statusPublished - Oct 10 2002

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Cite this

Saluz, H. P., Iqbal, J., Limmon, G. V., Ruryk, A., & Wu, Z. (2002). Fundamentals of DNA-chip/array technology for comparative gene-expression analysis. Current Science, 83(7), 829-833.