In this article we review the state-of-the-art of DNA-chip/array technology and its application in biological and medical sciences. With many of the technological hurdles to overcome array fabrication, many academic groups and numerous specialized companies are developing new tools and strategies for exploiting this promising field. DNA-microarrays are fabricated either by in situ synthesis or by conventional means, e.g. amplification of specific target DNA, followed by immobilization on the surface of various miniaturized substrates. The immobilized DNA molecules are hybridized with labelled probes. One single experiment can encompass aspects of many thousands of genes simultaneously. The unprecedented speed and parallelism of this technology has begun to have profound impacts on biological, environmental, pharmacological and medical research.
|Original language||English (US)|
|Number of pages||5|
|Publication status||Published - Oct 10 2002|
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