Fundamental contribution of β-oxidation to polyketide mycotoxin production in planta

Lori A. Maggio-Hall, Richard A. Wilson, Nancy P. Keller

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Seed contamination with polyketide mycotoxins, including aflatoxin (AF) and sterigmatocystin (ST) produced by Aspergillus spp., is an agricultural, economic, and medical issue worldwide. Acetyl-CoA, the fundamental building block of all known fungal polyketides, is generated by a large number of biochemical pathways, including β-oxidation of fatty acids and glycolysis of sugars. We present several lines of evidence to support a major role for seed fatty acids in formation of AF and ST in A. flavus, A. parasiticus, and A. nidulans. Aspergillus strains exhibiting canonical signs of oleic acid-induced peroxisome proliferation, including increased catalase activity, β-oxidation gene expression, and peroxisomal clustering, also exhibited a marked increase in toxin gene expression and biosynthesis. Furthermore, microscopic observations showed that the ST and AF precursor norsolorinic acid accumulated in peroxisomes of all three Aspergilli. While a peroxisomal β-oxidation mutation eliminated oleic acid-induced increases in ST in A. nidulans, a mitochondrial β-oxidation mutation played a larger role in eliminating ST formation on oatmeal medium and on live corn kernels, implicating a fundamental role for both peroxisomal and mitochondrial β-oxidation in toxin production.

Original languageEnglish (US)
Pages (from-to)783-793
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2005

Fingerprint

Sterigmatocystin
sterigmatocystin
Polyketides
polyketides
Mycotoxins
Plantae
mycotoxins
Aflatoxins
oxidation
Aspergillus
Oxidation
aflatoxins
Peroxisomes
peroxisomes
Oleic Acid
Gene expression
oleic acid
Seed
Seeds
toxins

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Fundamental contribution of β-oxidation to polyketide mycotoxin production in planta. / Maggio-Hall, Lori A.; Wilson, Richard A.; Keller, Nancy P.

In: Molecular Plant-Microbe Interactions, Vol. 18, No. 8, 01.08.2005, p. 783-793.

Research output: Contribution to journalArticle

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