Functions of human DNA polymerases η, κ and ι suggested by their properties, including fidelity with undamaged DNA templates

Thomas A. Kunkel, Youri I. Pavlov, Katarzyna Bebenek

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Human DNA polymerases η, κ and ι are template-dependent, Y-family DNA polymerases that have been implicated in translesion DNA synthesis (TLS) in human cells. Here, we briefly review evidence that these exonuclease-deficient polymerases copy undamaged DNA with very low fidelity and unusual error specificity. Based on the base substitution specificity and other biochemical properties of DNA polymerases η and ι, we consider the possibility that they participate in specialized DNA transactions that repair damaged DNA and/or generate mutations in the variable regions of immunoglobulin genes.

Original languageEnglish (US)
Pages (from-to)135-149
Number of pages15
JournalDNA Repair
Volume2
Issue number2
DOIs
StatePublished - Feb 3 2003

Fingerprint

DNA-Directed DNA Polymerase
DNA
Exonucleases
Immunoglobulin Genes
Immunoglobulin Variable Region
DNA Repair
Mutation
Repair
Substitution reactions
Genes
Cells

Keywords

  • DNA replication fidelity
  • Somatic hypermutation
  • Y-family DNA polymerases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functions of human DNA polymerases η, κ and ι suggested by their properties, including fidelity with undamaged DNA templates. / Kunkel, Thomas A.; Pavlov, Youri I.; Bebenek, Katarzyna.

In: DNA Repair, Vol. 2, No. 2, 03.02.2003, p. 135-149.

Research output: Contribution to journalArticle

@article{75dabfbf7deb4d62b7dcf59f20af284c,
title = "Functions of human DNA polymerases η, κ and ι suggested by their properties, including fidelity with undamaged DNA templates",
abstract = "Human DNA polymerases η, κ and ι are template-dependent, Y-family DNA polymerases that have been implicated in translesion DNA synthesis (TLS) in human cells. Here, we briefly review evidence that these exonuclease-deficient polymerases copy undamaged DNA with very low fidelity and unusual error specificity. Based on the base substitution specificity and other biochemical properties of DNA polymerases η and ι, we consider the possibility that they participate in specialized DNA transactions that repair damaged DNA and/or generate mutations in the variable regions of immunoglobulin genes.",
keywords = "DNA replication fidelity, Somatic hypermutation, Y-family DNA polymerases",
author = "Kunkel, {Thomas A.} and Pavlov, {Youri I.} and Katarzyna Bebenek",
year = "2003",
month = "2",
day = "3",
doi = "10.1016/S1568-7864(02)00224-0",
language = "English (US)",
volume = "2",
pages = "135--149",
journal = "DNA Repair",
issn = "1568-7864",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Functions of human DNA polymerases η, κ and ι suggested by their properties, including fidelity with undamaged DNA templates

AU - Kunkel, Thomas A.

AU - Pavlov, Youri I.

AU - Bebenek, Katarzyna

PY - 2003/2/3

Y1 - 2003/2/3

N2 - Human DNA polymerases η, κ and ι are template-dependent, Y-family DNA polymerases that have been implicated in translesion DNA synthesis (TLS) in human cells. Here, we briefly review evidence that these exonuclease-deficient polymerases copy undamaged DNA with very low fidelity and unusual error specificity. Based on the base substitution specificity and other biochemical properties of DNA polymerases η and ι, we consider the possibility that they participate in specialized DNA transactions that repair damaged DNA and/or generate mutations in the variable regions of immunoglobulin genes.

AB - Human DNA polymerases η, κ and ι are template-dependent, Y-family DNA polymerases that have been implicated in translesion DNA synthesis (TLS) in human cells. Here, we briefly review evidence that these exonuclease-deficient polymerases copy undamaged DNA with very low fidelity and unusual error specificity. Based on the base substitution specificity and other biochemical properties of DNA polymerases η and ι, we consider the possibility that they participate in specialized DNA transactions that repair damaged DNA and/or generate mutations in the variable regions of immunoglobulin genes.

KW - DNA replication fidelity

KW - Somatic hypermutation

KW - Y-family DNA polymerases

UR - http://www.scopus.com/inward/record.url?scp=0037415391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037415391&partnerID=8YFLogxK

U2 - 10.1016/S1568-7864(02)00224-0

DO - 10.1016/S1568-7864(02)00224-0

M3 - Article

C2 - 12531385

AN - SCOPUS:0037415391

VL - 2

SP - 135

EP - 149

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

IS - 2

ER -