Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness

Mohammad Saeid Jami, Jinxuan Hou, Miao Liu, Michelle L. Varney, Hesham Hassan, Jixin Dong, Liying Geng, Jing Wang, Fang Yu, Xin Huang, Hong Peng, Kai Fu, Yan Li, Rakesh K Singh, Shi Jian Ding

Research output: Contribution to journalArticle

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Abstract

Background: KIAA1199 is a recently identified novel gene that is up-regulated in human cancer with poor survival. Our proteomic study on signaling polarity in chemotactic cells revealed KIAA1199 as a novel protein target that may be involved in cellular chemotaxis and motility. In the present study, we examined the functional significance of KIAA1199 expression in breast cancer growth, motility and invasiveness.Methods: We validated the previous microarray observation by tissue microarray immunohistochemistry using a TMA slide containing 12 breast tumor tissue cores and 12 corresponding normal tissues. We performed the shRNA-mediated knockdown of KIAA1199 in MDA-MB-231 and HS578T cells to study the role of this protein in cell proliferation, migration and apoptosis in vitro. We studied the effects of KIAA1199 knockdown in vivo in two groups of mice (n = 5). We carried out the SILAC LC-MS/MS based proteomic studies on the involvement of KIAA1199 in breast cancer.Results: KIAA1199 mRNA and protein was significantly overexpressed in breast tumor specimens and cell lines as compared with non-neoplastic breast tissues from large-scale microarray and studies of breast cancer cell lines and tumors. To gain deeper insights into the novel role of KIAA1199 in breast cancer, we modulated KIAA1199 expression using shRNA-mediated knockdown in two breast cancer cell lines (MDA-MB-231 and HS578T), expressing higher levels of KIAA1199. The KIAA1199 knockdown cells showed reduced motility and cell proliferation in vitro. Moreover, when the knockdown cells were injected into the mammary fat pads of female athymic nude mice, there was a significant decrease in tumor incidence and growth. In addition, quantitative proteomic analysis revealed that knockdown of KIAA1199 in breast cancer (MDA-MB-231) cells affected a broad range of cellular functions including apoptosis, metabolism and cell motility.Conclusions: Our findings indicate that KIAA1199 may play an important role in breast tumor growth and invasiveness, and that it may represent a novel target for biomarker development and a novel therapeutic target for breast cancer.

Original languageEnglish (US)
Article number194
JournalBMC cancer
Volume14
Issue number1
DOIs
StatePublished - Mar 15 2014

Fingerprint

Proteomics
Breast Neoplasms
Growth
Tumor Cell Line
Nude Mice
Small Interfering RNA
Cell Movement
Breast
Cell Proliferation
Apoptosis
Proteins
Chemotaxis
Adipose Tissue
Neoplasms
Biomarkers
Immunohistochemistry
Observation
Cell Line
Messenger RNA
Survival

Keywords

  • Breast cancer
  • KIAA1199
  • Quantitative proteomic analysis

ASJC Scopus subject areas

  • Genetics
  • Oncology
  • Cancer Research

Cite this

Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness. / Jami, Mohammad Saeid; Hou, Jinxuan; Liu, Miao; Varney, Michelle L.; Hassan, Hesham; Dong, Jixin; Geng, Liying; Wang, Jing; Yu, Fang; Huang, Xin; Peng, Hong; Fu, Kai; Li, Yan; Singh, Rakesh K; Ding, Shi Jian.

In: BMC cancer, Vol. 14, No. 1, 194, 15.03.2014.

Research output: Contribution to journalArticle

Jami, MS, Hou, J, Liu, M, Varney, ML, Hassan, H, Dong, J, Geng, L, Wang, J, Yu, F, Huang, X, Peng, H, Fu, K, Li, Y, Singh, RK & Ding, SJ 2014, 'Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness', BMC cancer, vol. 14, no. 1, 194. https://doi.org/10.1186/1471-2407-14-194
Jami, Mohammad Saeid ; Hou, Jinxuan ; Liu, Miao ; Varney, Michelle L. ; Hassan, Hesham ; Dong, Jixin ; Geng, Liying ; Wang, Jing ; Yu, Fang ; Huang, Xin ; Peng, Hong ; Fu, Kai ; Li, Yan ; Singh, Rakesh K ; Ding, Shi Jian. / Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness. In: BMC cancer. 2014 ; Vol. 14, No. 1.
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abstract = "Background: KIAA1199 is a recently identified novel gene that is up-regulated in human cancer with poor survival. Our proteomic study on signaling polarity in chemotactic cells revealed KIAA1199 as a novel protein target that may be involved in cellular chemotaxis and motility. In the present study, we examined the functional significance of KIAA1199 expression in breast cancer growth, motility and invasiveness.Methods: We validated the previous microarray observation by tissue microarray immunohistochemistry using a TMA slide containing 12 breast tumor tissue cores and 12 corresponding normal tissues. We performed the shRNA-mediated knockdown of KIAA1199 in MDA-MB-231 and HS578T cells to study the role of this protein in cell proliferation, migration and apoptosis in vitro. We studied the effects of KIAA1199 knockdown in vivo in two groups of mice (n = 5). We carried out the SILAC LC-MS/MS based proteomic studies on the involvement of KIAA1199 in breast cancer.Results: KIAA1199 mRNA and protein was significantly overexpressed in breast tumor specimens and cell lines as compared with non-neoplastic breast tissues from large-scale microarray and studies of breast cancer cell lines and tumors. To gain deeper insights into the novel role of KIAA1199 in breast cancer, we modulated KIAA1199 expression using shRNA-mediated knockdown in two breast cancer cell lines (MDA-MB-231 and HS578T), expressing higher levels of KIAA1199. The KIAA1199 knockdown cells showed reduced motility and cell proliferation in vitro. Moreover, when the knockdown cells were injected into the mammary fat pads of female athymic nude mice, there was a significant decrease in tumor incidence and growth. In addition, quantitative proteomic analysis revealed that knockdown of KIAA1199 in breast cancer (MDA-MB-231) cells affected a broad range of cellular functions including apoptosis, metabolism and cell motility.Conclusions: Our findings indicate that KIAA1199 may play an important role in breast tumor growth and invasiveness, and that it may represent a novel target for biomarker development and a novel therapeutic target for breast cancer.",
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AU - Jami, Mohammad Saeid

AU - Hou, Jinxuan

AU - Liu, Miao

AU - Varney, Michelle L.

AU - Hassan, Hesham

AU - Dong, Jixin

AU - Geng, Liying

AU - Wang, Jing

AU - Yu, Fang

AU - Huang, Xin

AU - Peng, Hong

AU - Fu, Kai

AU - Li, Yan

AU - Singh, Rakesh K

AU - Ding, Shi Jian

PY - 2014/3/15

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N2 - Background: KIAA1199 is a recently identified novel gene that is up-regulated in human cancer with poor survival. Our proteomic study on signaling polarity in chemotactic cells revealed KIAA1199 as a novel protein target that may be involved in cellular chemotaxis and motility. In the present study, we examined the functional significance of KIAA1199 expression in breast cancer growth, motility and invasiveness.Methods: We validated the previous microarray observation by tissue microarray immunohistochemistry using a TMA slide containing 12 breast tumor tissue cores and 12 corresponding normal tissues. We performed the shRNA-mediated knockdown of KIAA1199 in MDA-MB-231 and HS578T cells to study the role of this protein in cell proliferation, migration and apoptosis in vitro. We studied the effects of KIAA1199 knockdown in vivo in two groups of mice (n = 5). We carried out the SILAC LC-MS/MS based proteomic studies on the involvement of KIAA1199 in breast cancer.Results: KIAA1199 mRNA and protein was significantly overexpressed in breast tumor specimens and cell lines as compared with non-neoplastic breast tissues from large-scale microarray and studies of breast cancer cell lines and tumors. To gain deeper insights into the novel role of KIAA1199 in breast cancer, we modulated KIAA1199 expression using shRNA-mediated knockdown in two breast cancer cell lines (MDA-MB-231 and HS578T), expressing higher levels of KIAA1199. The KIAA1199 knockdown cells showed reduced motility and cell proliferation in vitro. Moreover, when the knockdown cells were injected into the mammary fat pads of female athymic nude mice, there was a significant decrease in tumor incidence and growth. In addition, quantitative proteomic analysis revealed that knockdown of KIAA1199 in breast cancer (MDA-MB-231) cells affected a broad range of cellular functions including apoptosis, metabolism and cell motility.Conclusions: Our findings indicate that KIAA1199 may play an important role in breast tumor growth and invasiveness, and that it may represent a novel target for biomarker development and a novel therapeutic target for breast cancer.

AB - Background: KIAA1199 is a recently identified novel gene that is up-regulated in human cancer with poor survival. Our proteomic study on signaling polarity in chemotactic cells revealed KIAA1199 as a novel protein target that may be involved in cellular chemotaxis and motility. In the present study, we examined the functional significance of KIAA1199 expression in breast cancer growth, motility and invasiveness.Methods: We validated the previous microarray observation by tissue microarray immunohistochemistry using a TMA slide containing 12 breast tumor tissue cores and 12 corresponding normal tissues. We performed the shRNA-mediated knockdown of KIAA1199 in MDA-MB-231 and HS578T cells to study the role of this protein in cell proliferation, migration and apoptosis in vitro. We studied the effects of KIAA1199 knockdown in vivo in two groups of mice (n = 5). We carried out the SILAC LC-MS/MS based proteomic studies on the involvement of KIAA1199 in breast cancer.Results: KIAA1199 mRNA and protein was significantly overexpressed in breast tumor specimens and cell lines as compared with non-neoplastic breast tissues from large-scale microarray and studies of breast cancer cell lines and tumors. To gain deeper insights into the novel role of KIAA1199 in breast cancer, we modulated KIAA1199 expression using shRNA-mediated knockdown in two breast cancer cell lines (MDA-MB-231 and HS578T), expressing higher levels of KIAA1199. The KIAA1199 knockdown cells showed reduced motility and cell proliferation in vitro. Moreover, when the knockdown cells were injected into the mammary fat pads of female athymic nude mice, there was a significant decrease in tumor incidence and growth. In addition, quantitative proteomic analysis revealed that knockdown of KIAA1199 in breast cancer (MDA-MB-231) cells affected a broad range of cellular functions including apoptosis, metabolism and cell motility.Conclusions: Our findings indicate that KIAA1199 may play an important role in breast tumor growth and invasiveness, and that it may represent a novel target for biomarker development and a novel therapeutic target for breast cancer.

KW - Breast cancer

KW - KIAA1199

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