Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody

Shi-Hua Xiang, Michael Farzan, Zhihai Si, Navid Madani, Liping Wang, Eric Rosenberg, James Robinson, Joseph Sodroski

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Interaction of the human immunodeficiency virus type 1 (HIV-1) gp120 envelope glycoprotein with the primary receptor, CD4, promotes binding to a chemokine receptor, either CCR5 or CXCR4. The chemokine receptor-binding site on gp120 elicits CD4-induced (CD4i) antibodies in some HIV-1-infected individuals. Like CCR5 itself, the CD4i antibody 412d exhibits a preference for CCR5-using HIV-1 strains and utilizes sulfated tyrosines to achieve binding to gp120. Here, we show that 412d binding requires the gp120 β19 strand and the base of the V3 loop, elements that are important for the binding of the CCR5 N terminus. Two gp120 residues in the V3 loop base determined 412d preference for CCR5-using HIV-1 strains. A chimeric molecule in which the 412d heavy-chain third complementarity-determining loop sequence replaces the CCR5 N terminus functioned as an efficient second receptor, selectively supporting the entry of CCR5-using HIV-1 strains. Sulfation of N-terminal tyrosines contributed to the function of this chimeric receptor. These results emphasize the close mimicry of the CCR5 N terminus by the gp120-interactive region of a naturally elicited CD4i antibody.

Original languageEnglish (US)
Pages (from-to)6068-6077
Number of pages10
JournalJournal of virology
Volume79
Issue number10
DOIs
StatePublished - May 1 2005

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Human immunodeficiency virus 1
Neutralizing Antibodies
neutralizing antibodies
HIV-1
monoclonal antibodies
Monoclonal Antibodies
Chemokine Receptors
receptors
antibodies
Tyrosine
tyrosine
Antibodies
CD4 Antigens
glycoproteins
binding sites
Glycoproteins
Binding Sites
chemokine receptors

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody. / Xiang, Shi-Hua; Farzan, Michael; Si, Zhihai; Madani, Navid; Wang, Liping; Rosenberg, Eric; Robinson, James; Sodroski, Joseph.

In: Journal of virology, Vol. 79, No. 10, 01.05.2005, p. 6068-6077.

Research output: Contribution to journalArticle

Xiang, S-H, Farzan, M, Si, Z, Madani, N, Wang, L, Rosenberg, E, Robinson, J & Sodroski, J 2005, 'Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody', Journal of virology, vol. 79, no. 10, pp. 6068-6077. https://doi.org/10.1128/JVI.79.10.6068-6077.2005
Xiang, Shi-Hua ; Farzan, Michael ; Si, Zhihai ; Madani, Navid ; Wang, Liping ; Rosenberg, Eric ; Robinson, James ; Sodroski, Joseph. / Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody. In: Journal of virology. 2005 ; Vol. 79, No. 10. pp. 6068-6077.
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