Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain

Jinju Han, Hyung Joon Kim, Simon T. Schafer, Apua Paquola, Gregory D. Clemenson, Tomohisa Toda, Jinseo Oh, Aimee R. Pankonin, Bo Suk Lee, Stephen T. Johnston, Anindita Sarkar, Ahmet M. Denli, Fred H. Gage

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Altered microRNA profiles have been implicated in human brain disorders. However, the functional contribution of individual microRNAs to neuronal development and function is largely unknown. Here, we report biological functions for miR-19 in adult neurogenesis. We determined that miR-19 is enriched in neural progenitor cells (NPCs) and downregulated during neuronal development in the adult hippocampus. By manipulating miR-19 in NPCs for gain- and loss-of-function studies, we discovered that miR-19 regulates cell migration by directly targeting Rapgef2. Concordantly, dysregulation of miR-19 in NPCs alters the positioning of newborn neurons in the adult brain. Furthermore, we found abnormal expression of miR-19 in human NPCs generated from schizophrenic patient-derived induced pluripotent stem cells (iPSCs) that have been described as displaying aberrant migration. Our study demonstrates the significance of posttranscriptional gene regulation by miR-19 in preventing the irregular migration of adult-born neurons that may contribute to the etiology of schizophrenia.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalNeuron
Volume91
Issue number1
DOIs
StatePublished - Jul 6 2016

Fingerprint

Stem Cells
Newborn Infant
Neurons
Brain
MicroRNAs
Induced Pluripotent Stem Cells
Neurogenesis
Brain Diseases
Cell Movement
Hippocampus
Schizophrenia
Down-Regulation
Genes

Keywords

  • Rapgef2
  • adult neurogenesis
  • cell migration
  • hippocampus
  • human induced pluripotent stem cells (iPSCs)
  • miR-19
  • microRNA (miRNA)
  • microRNA in vivo functions
  • neural progenitor cells (NPCs)
  • polycistronic microRNAs
  • schizophrenia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Han, J., Kim, H. J., Schafer, S. T., Paquola, A., Clemenson, G. D., Toda, T., ... Gage, F. H. (2016). Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain. Neuron, 91(1), 79-89. https://doi.org/10.1016/j.neuron.2016.05.034

Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain. / Han, Jinju; Kim, Hyung Joon; Schafer, Simon T.; Paquola, Apua; Clemenson, Gregory D.; Toda, Tomohisa; Oh, Jinseo; Pankonin, Aimee R.; Lee, Bo Suk; Johnston, Stephen T.; Sarkar, Anindita; Denli, Ahmet M.; Gage, Fred H.

In: Neuron, Vol. 91, No. 1, 06.07.2016, p. 79-89.

Research output: Contribution to journalArticle

Han, J, Kim, HJ, Schafer, ST, Paquola, A, Clemenson, GD, Toda, T, Oh, J, Pankonin, AR, Lee, BS, Johnston, ST, Sarkar, A, Denli, AM & Gage, FH 2016, 'Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain', Neuron, vol. 91, no. 1, pp. 79-89. https://doi.org/10.1016/j.neuron.2016.05.034
Han, Jinju ; Kim, Hyung Joon ; Schafer, Simon T. ; Paquola, Apua ; Clemenson, Gregory D. ; Toda, Tomohisa ; Oh, Jinseo ; Pankonin, Aimee R. ; Lee, Bo Suk ; Johnston, Stephen T. ; Sarkar, Anindita ; Denli, Ahmet M. ; Gage, Fred H. / Functional Implications of miR-19 in the Migration of Newborn Neurons in the Adult Brain. In: Neuron. 2016 ; Vol. 91, No. 1. pp. 79-89.
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