Functional implication of the tRNA genes encoded in the Chlorella virus PBCV-1 genome

Da Young Lee, Michael V. Graves, James L. van Etten, Tae Jin Choi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The prototype Chlorella virus PBCV-1 encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the al89/192r gene showed highly biased usage of the AGA and AUA codons compared to genes encoded by E. coli and Chlorella. In addition, there were biases of XXA/U (56%) and XXG/ C (44%) in the codons recognized by the viral tRNAs, which correspond to the codon usage bias in the PBCV-1 genome of XXA/U (63%) over those ending in XXC/G (37%). Analysis of the codon usage in the major capsid protein and DNA polymerase showed preferential usage of codons that can be recognized by the viral tRNAs. The Asn (AAC) and Lys (AAG) codons whose corresponding tRNA genes are duplicated in the tRNA gene cluster were the most abundant (i.e., preferred) codons in these two proteins. The tRNA genes encoded in the PBCV-1 genome seem to play a very important role during the synthesis of viral proteins through supplementing the tRNAs that are frequently used in viral proteins, but are rare in the host cells. In addition, these tRNAs would help the virus to adapt to a wide range of hosts by providing tRNAs that are rare in the host cells.

Original languageEnglish (US)
Pages (from-to)334-342
Number of pages9
JournalPlant Pathology Journal
Volume21
Issue number4
DOIs
StatePublished - Dec 1 2005

Fingerprint

Paramecium bursaria Chlorella virus 1
Chlorella
codons
viruses
genome
genes
viral proteins
Escherichia coli
proteins
DNA-directed DNA polymerase
coat proteins
multigene family
recombinant proteins
prototypes
host range
Western blotting

Keywords

  • Adaptation
  • Chlorella virus
  • Codon bias
  • tRNA

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Functional implication of the tRNA genes encoded in the Chlorella virus PBCV-1 genome. / Lee, Da Young; Graves, Michael V.; van Etten, James L.; Choi, Tae Jin.

In: Plant Pathology Journal, Vol. 21, No. 4, 01.12.2005, p. 334-342.

Research output: Contribution to journalArticle

Lee, Da Young ; Graves, Michael V. ; van Etten, James L. ; Choi, Tae Jin. / Functional implication of the tRNA genes encoded in the Chlorella virus PBCV-1 genome. In: Plant Pathology Journal. 2005 ; Vol. 21, No. 4. pp. 334-342.
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abstract = "The prototype Chlorella virus PBCV-1 encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the al89/192r gene showed highly biased usage of the AGA and AUA codons compared to genes encoded by E. coli and Chlorella. In addition, there were biases of XXA/U (56{\%}) and XXG/ C (44{\%}) in the codons recognized by the viral tRNAs, which correspond to the codon usage bias in the PBCV-1 genome of XXA/U (63{\%}) over those ending in XXC/G (37{\%}). Analysis of the codon usage in the major capsid protein and DNA polymerase showed preferential usage of codons that can be recognized by the viral tRNAs. The Asn (AAC) and Lys (AAG) codons whose corresponding tRNA genes are duplicated in the tRNA gene cluster were the most abundant (i.e., preferred) codons in these two proteins. The tRNA genes encoded in the PBCV-1 genome seem to play a very important role during the synthesis of viral proteins through supplementing the tRNAs that are frequently used in viral proteins, but are rare in the host cells. In addition, these tRNAs would help the virus to adapt to a wide range of hosts by providing tRNAs that are rare in the host cells.",
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