Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses

Timo Greiner, José Ramos, Maria C. Alvarez, James R. Gurnon, Ming Kang, James L Van Etten, Anna Moroni, Gerhard Thiel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chlorella viruses are a source of interesting membrane transport proteins. Here we examine a putative K + transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence and structural features with HAK/KUP/KT-like K + transporters from plants, bacteria and fungi. Yeast complementation assays and Rb + uptake experiments show that the viral protein, termed HAKCV (high-affinity K + transporter of chlorella virus), is functional, with transport characteristics that are similar to those of known K + transporters. Expression studies revealed that the protein is expressed as an early gene during viral replication, and proteomics data indicate that it is not packaged in the virion. The function of HAKCV is unclear, but the data refute the hypothesis that the transporter acts as a substitute for viral-encoded K + channels during virus infection.

Original languageEnglish (US)
Pages (from-to)977-986
Number of pages10
JournalPlant Journal
Volume68
Issue number6
DOIs
StatePublished - Dec 1 2011

Fingerprint

Chlorella
transporters
Potassium
potassium
Viruses
viruses
Membrane Transport Proteins
Viral Genes
Viral Proteins
Virus Diseases
Virion
Proteomics
viral proteins
potassium channels
transport proteins
virus replication
virion
Proteins
membrane proteins
Fungi

Keywords

  • HAK/KUP/KT-like potassium transporter
  • HAK5
  • chlorella virus membrane proteins
  • viral K channel Kcv
  • virus K transporter

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Greiner, T., Ramos, J., Alvarez, M. C., Gurnon, J. R., Kang, M., Van Etten, J. L., ... Thiel, G. (2011). Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses. Plant Journal, 68(6), 977-986. https://doi.org/10.1111/j.1365-313X.2011.04748.x

Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses. / Greiner, Timo; Ramos, José; Alvarez, Maria C.; Gurnon, James R.; Kang, Ming; Van Etten, James L; Moroni, Anna; Thiel, Gerhard.

In: Plant Journal, Vol. 68, No. 6, 01.12.2011, p. 977-986.

Research output: Contribution to journalArticle

Greiner, T, Ramos, J, Alvarez, MC, Gurnon, JR, Kang, M, Van Etten, JL, Moroni, A & Thiel, G 2011, 'Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses', Plant Journal, vol. 68, no. 6, pp. 977-986. https://doi.org/10.1111/j.1365-313X.2011.04748.x
Greiner, Timo ; Ramos, José ; Alvarez, Maria C. ; Gurnon, James R. ; Kang, Ming ; Van Etten, James L ; Moroni, Anna ; Thiel, Gerhard. / Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses. In: Plant Journal. 2011 ; Vol. 68, No. 6. pp. 977-986.
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