Functional complementation of fumonisin biosynthesis in FUM1-disrupted Fusarium verticillioides by the AAL-toxin Polyketide synthase gene ALT1 from Alternaria alternata f. sp. Lycopersici

Xiangcheng Zhu, Chad Vogeler, Liangcheng Du

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fumonisins and AAL-toxins are mycotoxins produced by several widespread fungal pathogens of crops. The carbon backbone of the mycotoxins originates from a highly reduced, acyclic polyketide, a C18 chain for fumonisins and a C16 chain for AAL-toxins. Fungal reduced polyketides are assembled by iterative modular polyketide synthases (PKS), and their biosynthetic mechanism is not very clear. Here, we cloned the PKS gene, ALT1, from the tomato pathogen Alternaria alternata f. sp. Lycopersici and introduced it into Fusarium verticillioides 5777, which does not produce fumonisins due to a disrupted fumonisin PKS gene, FUM1. An ALT1 transformant of strain 5777 produced fumonisin B series as well as fumonisin analogues. The results provide experimental evidence for the function of ALT1, which encodes a PKS for mycotoxin biosynthesis. The results also show that the C16-synthesizing ALT1 is able to support the C18 fumonisin biosynthesis in F. verticillioides, suggesting that the final size of the fungal reduced polyketides is not determined by the PKSs alone. Unlike other PKSs, these PKSs do not have a thioesterase/cyclase domain. The release of polyketide precursors that are covalently attached to the PKSs involves a distinct mechanism, which probably determines the structure of the final products.

Original languageEnglish (US)
Pages (from-to)957-960
Number of pages4
JournalJournal of Natural Products
Volume71
Issue number6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Polyketide Synthases
Fumonisins
polyketide synthases
Alternaria
Alternaria alternata
fumonisins
Biosynthesis
Fusarium
toxins
Genes
Polyketides
polyketides
biosynthesis
Mycotoxins
mycotoxins
genes
Pathogens
pathogens
Lycopersicon esculentum
Alternaria alternata pathotoxin TA

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

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title = "Functional complementation of fumonisin biosynthesis in FUM1-disrupted Fusarium verticillioides by the AAL-toxin Polyketide synthase gene ALT1 from Alternaria alternata f. sp. Lycopersici",
abstract = "Fumonisins and AAL-toxins are mycotoxins produced by several widespread fungal pathogens of crops. The carbon backbone of the mycotoxins originates from a highly reduced, acyclic polyketide, a C18 chain for fumonisins and a C16 chain for AAL-toxins. Fungal reduced polyketides are assembled by iterative modular polyketide synthases (PKS), and their biosynthetic mechanism is not very clear. Here, we cloned the PKS gene, ALT1, from the tomato pathogen Alternaria alternata f. sp. Lycopersici and introduced it into Fusarium verticillioides 5777, which does not produce fumonisins due to a disrupted fumonisin PKS gene, FUM1. An ALT1 transformant of strain 5777 produced fumonisin B series as well as fumonisin analogues. The results provide experimental evidence for the function of ALT1, which encodes a PKS for mycotoxin biosynthesis. The results also show that the C16-synthesizing ALT1 is able to support the C18 fumonisin biosynthesis in F. verticillioides, suggesting that the final size of the fungal reduced polyketides is not determined by the PKSs alone. Unlike other PKSs, these PKSs do not have a thioesterase/cyclase domain. The release of polyketide precursors that are covalently attached to the PKSs involves a distinct mechanism, which probably determines the structure of the final products.",
author = "Xiangcheng Zhu and Chad Vogeler and Liangcheng Du",
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T1 - Functional complementation of fumonisin biosynthesis in FUM1-disrupted Fusarium verticillioides by the AAL-toxin Polyketide synthase gene ALT1 from Alternaria alternata f. sp. Lycopersici

AU - Zhu, Xiangcheng

AU - Vogeler, Chad

AU - Du, Liangcheng

PY - 2008/6/1

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N2 - Fumonisins and AAL-toxins are mycotoxins produced by several widespread fungal pathogens of crops. The carbon backbone of the mycotoxins originates from a highly reduced, acyclic polyketide, a C18 chain for fumonisins and a C16 chain for AAL-toxins. Fungal reduced polyketides are assembled by iterative modular polyketide synthases (PKS), and their biosynthetic mechanism is not very clear. Here, we cloned the PKS gene, ALT1, from the tomato pathogen Alternaria alternata f. sp. Lycopersici and introduced it into Fusarium verticillioides 5777, which does not produce fumonisins due to a disrupted fumonisin PKS gene, FUM1. An ALT1 transformant of strain 5777 produced fumonisin B series as well as fumonisin analogues. The results provide experimental evidence for the function of ALT1, which encodes a PKS for mycotoxin biosynthesis. The results also show that the C16-synthesizing ALT1 is able to support the C18 fumonisin biosynthesis in F. verticillioides, suggesting that the final size of the fungal reduced polyketides is not determined by the PKSs alone. Unlike other PKSs, these PKSs do not have a thioesterase/cyclase domain. The release of polyketide precursors that are covalently attached to the PKSs involves a distinct mechanism, which probably determines the structure of the final products.

AB - Fumonisins and AAL-toxins are mycotoxins produced by several widespread fungal pathogens of crops. The carbon backbone of the mycotoxins originates from a highly reduced, acyclic polyketide, a C18 chain for fumonisins and a C16 chain for AAL-toxins. Fungal reduced polyketides are assembled by iterative modular polyketide synthases (PKS), and their biosynthetic mechanism is not very clear. Here, we cloned the PKS gene, ALT1, from the tomato pathogen Alternaria alternata f. sp. Lycopersici and introduced it into Fusarium verticillioides 5777, which does not produce fumonisins due to a disrupted fumonisin PKS gene, FUM1. An ALT1 transformant of strain 5777 produced fumonisin B series as well as fumonisin analogues. The results provide experimental evidence for the function of ALT1, which encodes a PKS for mycotoxin biosynthesis. The results also show that the C16-synthesizing ALT1 is able to support the C18 fumonisin biosynthesis in F. verticillioides, suggesting that the final size of the fungal reduced polyketides is not determined by the PKSs alone. Unlike other PKSs, these PKSs do not have a thioesterase/cyclase domain. The release of polyketide precursors that are covalently attached to the PKSs involves a distinct mechanism, which probably determines the structure of the final products.

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