Functional characterization of the Aspergillus fumigatus calcineurin

Márcia Eliana da Silva Ferreira, Thorsten Heinekamp, Albert Härtl, Axel A. Brakhage, Camile P. Semighini, Steven D. Harris, Marcela Savoldi, Paula Fagundes de Gouvêa, Maria Helena de Souza Goldman, Gustavo H. Goldman

Research output: Contribution to journalArticle

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Abstract

Aspergillus fumigatus is an aggressive opportunistic pathogen of humans as well as a major allergen. Environmental sensing and retrieving essential nutrients from the environment are general metabolic traits associated with the growth of this saprophytic fungus. Two important mediators of calcium signals in eukaryotic cells are the Ca2+-binding protein calmodulin and the Ca2+/calmodulin-dependent phosphatase calcineurin. Calcineurin is a heterodimer that consists of a catalytic subunit A and a Ca2+/calmodulin binding unit. We deleted the A. fumigatus calA gene, which encodes the calcineurin A catalytic subunit, and demonstrated that this gene is not essential in this fungus. The ΔcalA mutant strain has severe defects in growth extension, branching and conidial architecture. Furthermore, the A. fumigatus ΔcalA mutant strain has decreased fitness in a low dose murine infection and cannot grow in fetal bovine serum (FBS). After potassium phosphate was added to liquid FBS, the ΔcalA mutant strain could grow with the characteristic phenotype of the ΔcalA mutation. When A. fumigatus calcineurin is inhibited by tacrolimus in a phosphate depleted medium, there is a reduction in the inorganic phosphate transport and six putative phosphate transporter genes have altered mRNA levels. However, there is no effect on the acid phosphatase activity. These results suggest that calcineurin is involved in the regulation of the PHO pathway in A. fumigatus. Our work on calcineurin opens new venues for the research on sensing and nutrient acquisition in A. fumigatus.

Original languageEnglish (US)
Pages (from-to)219-230
Number of pages12
JournalFungal Genetics and Biology
Volume44
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Aspergillus fumigatus
Calcineurin
Calmodulin
Catalytic Domain
Fungi
Phosphates
Phosphate Transport Proteins
Genes
Calmodulin-Binding Proteins
Tacrolimus
Eukaryotic Cells
Growth
Acid Phosphatase
Serum
Allergens
Calcium
Phenotype
Food
Messenger RNA
Mutation

Keywords

  • Aspergillus fumigatus
  • Calcineurin
  • Phosphate assimilation

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

Ferreira, M. E. D. S., Heinekamp, T., Härtl, A., Brakhage, A. A., Semighini, C. P., Harris, S. D., ... Goldman, G. H. (2007). Functional characterization of the Aspergillus fumigatus calcineurin. Fungal Genetics and Biology, 44(3), 219-230. https://doi.org/10.1016/j.fgb.2006.08.004

Functional characterization of the Aspergillus fumigatus calcineurin. / Ferreira, Márcia Eliana da Silva; Heinekamp, Thorsten; Härtl, Albert; Brakhage, Axel A.; Semighini, Camile P.; Harris, Steven D.; Savoldi, Marcela; de Gouvêa, Paula Fagundes; Goldman, Maria Helena de Souza; Goldman, Gustavo H.

In: Fungal Genetics and Biology, Vol. 44, No. 3, 01.03.2007, p. 219-230.

Research output: Contribution to journalArticle

Ferreira, MEDS, Heinekamp, T, Härtl, A, Brakhage, AA, Semighini, CP, Harris, SD, Savoldi, M, de Gouvêa, PF, Goldman, MHDS & Goldman, GH 2007, 'Functional characterization of the Aspergillus fumigatus calcineurin', Fungal Genetics and Biology, vol. 44, no. 3, pp. 219-230. https://doi.org/10.1016/j.fgb.2006.08.004
Ferreira MEDS, Heinekamp T, Härtl A, Brakhage AA, Semighini CP, Harris SD et al. Functional characterization of the Aspergillus fumigatus calcineurin. Fungal Genetics and Biology. 2007 Mar 1;44(3):219-230. https://doi.org/10.1016/j.fgb.2006.08.004
Ferreira, Márcia Eliana da Silva ; Heinekamp, Thorsten ; Härtl, Albert ; Brakhage, Axel A. ; Semighini, Camile P. ; Harris, Steven D. ; Savoldi, Marcela ; de Gouvêa, Paula Fagundes ; Goldman, Maria Helena de Souza ; Goldman, Gustavo H. / Functional characterization of the Aspergillus fumigatus calcineurin. In: Fungal Genetics and Biology. 2007 ; Vol. 44, No. 3. pp. 219-230.
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