Functional characterization of the Arabidopsis β-ketoacyl-coenzyme a reductase candidates of the fatty acid elongase

Frédéric Beaudoin, Xianzhong Wu, Fengling Li, Richard P. Haslam, Jonathan E. Markham, Huanquan Zheng, Johnathan A. Napier, Ljerka Kunst

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

In plants, very-long-chain fatty acids (VLCFAs;>8 carbon) are precursors of sphingolipids, triacylglycerols, cuticular waxes, and suberin. VLCFAs are synthesized by a multiprotein membrane-bound fatty acid elongation system that catalyzes four successive enzymatic reactions: condensation, reduction, dehydration, and a second reduction. A bioinformatics survey of the Arabidopsis (Arabidopsis thaliana) genome has revealed two sequences homologous to YBR159w encoding a Saccharomyces cerevisiae b-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA elongation. Expression analyses showed that both AtKCR1 and AtKCR2 genes were transcribed in siliques, flowers, inflorescence stems, leaves, as well as developing embryos, but only AtKCR1 transcript was detected in roots. Fluorescent protein-tagged AtKCR1 and AtKCR2 were localized to the endoplasmic reticulum, the site of fatty acid elongation. Complementation of the yeast ybr159D mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene. Analyses of insertional mutants in AtKCR1 and AtKCR2 revealed that loss of AtKCR1 function results in embryo lethality, which cannot be rescued by AtKCR2 expression using the AtKCR1 promoter. In contrast, a disruption of the AtKCR2 gene had no obvious phenotypic effect. Taken together, these results indicate that only AtKCR1 is a functional KCR isoform involved in microsomal fatty acid elongation. To investigate the roles of AtKCR1 in postembryonic development, transgenic lines expressing RNA interference and overexpression constructs targeted against AtKCR1 were generated. Morphological and biochemical characterization of these lines confirmed that suppressed KCR activity results in a reduction of cuticular wax load and affects VLCFA composition of sphingolipids, seed triacylglycerols, and root glycerolipids, demonstrating in planta that KCR is involved in elongation reactions supplying VLCFA for all these diverse classes of lipids.

Original languageEnglish (US)
Pages (from-to)1174-1191
Number of pages18
JournalPlant physiology
Volume150
Issue number3
DOIs
StatePublished - Jul 1 2009

Fingerprint

very long chain fatty acids
coenzymes
Coenzymes
Arabidopsis
Oxidoreductases
fatty acids
Fatty Acids
sphingolipids
Sphingolipids
epicuticular wax
Waxes
embryo (plant)
Triglycerides
Embryonic Structures
Yeasts
triacylglycerols
Genes
yeasts
suberin
Inflorescence

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Functional characterization of the Arabidopsis β-ketoacyl-coenzyme a reductase candidates of the fatty acid elongase. / Beaudoin, Frédéric; Wu, Xianzhong; Li, Fengling; Haslam, Richard P.; Markham, Jonathan E.; Zheng, Huanquan; Napier, Johnathan A.; Kunst, Ljerka.

In: Plant physiology, Vol. 150, No. 3, 01.07.2009, p. 1174-1191.

Research output: Contribution to journalArticle

Beaudoin, Frédéric ; Wu, Xianzhong ; Li, Fengling ; Haslam, Richard P. ; Markham, Jonathan E. ; Zheng, Huanquan ; Napier, Johnathan A. ; Kunst, Ljerka. / Functional characterization of the Arabidopsis β-ketoacyl-coenzyme a reductase candidates of the fatty acid elongase. In: Plant physiology. 2009 ; Vol. 150, No. 3. pp. 1174-1191.
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AU - Zheng, Huanquan

AU - Napier, Johnathan A.

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