Functional C5a receptors on murine bone marrow-derived stromal cells enhance localization of a murine lympho-hematopoietic cell line

L. A. Welniak, S. Sanderson, L. Clark-Pierce, J. D. Jackson

Research output: Contribution to journalArticle

Abstract

C5a anaphylatoxin, a 74-residue glycopoly pep tide generated as a cleavage product of the fifth component of complement (C5), is known to be a potent activator and chemotactic agent of monocytes and neutrophils. To determine if C5a can affect the interactions of lympho-hematopoietic cells with the bone marrow stromal microenvironment, a cloned murine stromal-dependent pro-B cell line, TAJ.B11, and a cloned murine stromal cell line, W/W2.G5, were used. Under normal growth conditions a portion of TAJ.B11 cells migrate under the stromal cell line. The addition of C5a or C5a decapeptide agonist had no effect on the number of migrating TAJ.B11 cells(89% ± 8 SEM migrating cells of untreated control). Pretreatment of the stromal cell line with recombinant human IL-1β resulted in a decrease in the number of migrating TAJ.B11 cells (63% ± 6). However, the addition of C5a or agonist peptide to the IL-1β pretreated stromal cells resulted in a significant increase in the number of migrating TAJ.B11 cells (223% ± 21 of IL-1 treated control). C5a receptors on murine and human stromal cells were demonstrated by flow cytometry and western blots. IL-1bβ pretreatment of the stromal cells did not lead to a significant change in the expression of C5aR, The results show that C5aR is constitutively expressed on bone marrow derived stromal cells and that C5a can enhance migration of a hematopoietic progenitor cell line following activation of the receptor on the stromal cells.

Original languageEnglish (US)
Pages (from-to)A115
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997

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Anaphylatoxin C5a Receptor
stromal cells
Stromal Cells
Mesenchymal Stromal Cells
bone marrow
Bone
Cells
cell lines
Cell Line
receptors
mice
Interleukin-1
interleukin-1
cells
Complement C5
Anaphylatoxins
agonists
Flow cytometry
pretreatment
Tides

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Functional C5a receptors on murine bone marrow-derived stromal cells enhance localization of a murine lympho-hematopoietic cell line. / Welniak, L. A.; Sanderson, S.; Clark-Pierce, L.; Jackson, J. D.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997, p. A115.

Research output: Contribution to journalArticle

Welniak, L. A. ; Sanderson, S. ; Clark-Pierce, L. ; Jackson, J. D. / Functional C5a receptors on murine bone marrow-derived stromal cells enhance localization of a murine lympho-hematopoietic cell line. In: FASEB Journal. 1997 ; Vol. 11, No. 3. pp. A115.
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