Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus

Rodolphe Barrangou, Eric Altermann, Robert Hutkins, Raul Cano, Todd R. Klaenhammer

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate the growth of beneficial commensals in the gastrointestinal tract. However, little is known about the mechanisms and genes involved in FOS utilization by Lactobacillus species. Analysis of the L. acidophilus NCFM genome revealed an msm locus composed of a transcriptional regulator of the Lacl family, a four-component ATP-binding cassette (ABC) transport system, a fructosidase, and a sucrose phosphorylase. Transcriptional analysis of this operon demonstrated that gene expression was induced by sucrose and FOS but not by glucose or fructose, suggesting some specificity for nonreadily fermentable sugars. Additionally, expression was repressed by glucose but not by fructose, suggesting catabolite repression via two cre-like sequences identified in the promoter-operator region. Insertional inactivation of the genes encoding the ABC transporter substrate-binding protein and the fructosidase reduced the ability of the mutants to grow on FOS. Comparative analysis of gene architecture within this cluster revealed a high degree of synteny with operons in Streptococcus mutans and Streptococcus pneumoniae. However, the association between a fructosidase and an ABC transporter is unusual and may be specific to L. acidophilus. This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)8957-8962
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number15
DOIs
StatePublished - Jul 22 2003

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Lactobacillus acidophilus
Operon
ATP-Binding Cassette Transporters
sucrose phosphorylase
Fructose
Gastrointestinal Tract
Genes
Catabolite Repression
Synteny
Glucose
Prebiotics
Streptococcus mutans
Probiotics
Gene Silencing
Lactobacillus
Streptococcus pneumoniae
Genetic Promoter Regions
Sucrose
Carrier Proteins
Adenosine Triphosphate

ASJC Scopus subject areas

  • General

Cite this

Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus. / Barrangou, Rodolphe; Altermann, Eric; Hutkins, Robert; Cano, Raul; Klaenhammer, Todd R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 15, 22.07.2003, p. 8957-8962.

Research output: Contribution to journalArticle

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