Functional analysis of the seven in absentia ubiquitin ligase family in tomato

Wenjie Wang, Youhong Fan, Xiangli Niu, Min Miao, Joanna Kud, Bangjun Zhou, Lirong Zeng, Yongsheng Liu, Fangming Xiao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Seven in absentia (SINA) protein is one subgroup of ubiquitin ligases possessing an N-terminal cysteine-rich really interesting new gene (RING) domain, two zinc-finger motifs, and a C-terminal domain responsible for substrate-binding and dimerization. In tomato (Solanum lycopersicum), the SINA gene family has six members, and we characterize in this study all tomato SINA (SlSINA) genes and the gene products. Our results show that SlSINA genes are differentially regulated in leaf, bud, stem, flower, and root. All SlSINA proteins possess RING-dependent E3 ubiquitin ligase activity, exhibiting similar specificity towards the E2 ubiquitin-conjugating enzyme. SlSINA1/3/4/5/6 are localized in both cytoplasm and nucleus, whereas SlSINA2 is exclusively localized in the nucleus. Moreover, all SlSINAs can interact with each other for homo- or hetero-dimerization. The functionality of SlSINA proteins has been investigated. SlSINA4 plays a positive role in defense signalling, as manifested by elicitation of E3-dependent hypersensitive response-like cell death; the other SlSINAs are negative regulator and capable to suppress hypersensitive response cell death. Transgenic tomato plants overexpressing SlSINA2 exhibit pale-green leaf phenotype, suggesting SlSINA2 regulates chlorophyll level in plant cells, whereas transgenic tomato plants overexpressing SlSINA5 have altered floral structure with exserted stigma, implicating SlSINA5 plays a role in flower development.

Original languageEnglish (US)
Pages (from-to)689-703
Number of pages15
JournalPlant Cell and Environment
Volume41
Issue number3
DOIs
StatePublished - Mar 2018

Fingerprint

Lycopersicon esculentum
Ligases
ubiquitin
Ubiquitin
ligases
tomatoes
dimerization
Dimerization
ubiquitin-protein ligase
Genes
genes
hypersensitive response
Genetically Modified Plants
cell death
genetically modified organisms
Cell Death
Ubiquitin-Conjugating Enzymes
proteins
zinc finger motif
Homo

Keywords

  • NAC transcription factor
  • UPS-mediated degradation
  • plant defense response

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Wang, W., Fan, Y., Niu, X., Miao, M., Kud, J., Zhou, B., ... Xiao, F. (2018). Functional analysis of the seven in absentia ubiquitin ligase family in tomato. Plant Cell and Environment, 41(3), 689-703. https://doi.org/10.1111/pce.13140

Functional analysis of the seven in absentia ubiquitin ligase family in tomato. / Wang, Wenjie; Fan, Youhong; Niu, Xiangli; Miao, Min; Kud, Joanna; Zhou, Bangjun; Zeng, Lirong; Liu, Yongsheng; Xiao, Fangming.

In: Plant Cell and Environment, Vol. 41, No. 3, 03.2018, p. 689-703.

Research output: Contribution to journalArticle

Wang, W, Fan, Y, Niu, X, Miao, M, Kud, J, Zhou, B, Zeng, L, Liu, Y & Xiao, F 2018, 'Functional analysis of the seven in absentia ubiquitin ligase family in tomato', Plant Cell and Environment, vol. 41, no. 3, pp. 689-703. https://doi.org/10.1111/pce.13140
Wang, Wenjie ; Fan, Youhong ; Niu, Xiangli ; Miao, Min ; Kud, Joanna ; Zhou, Bangjun ; Zeng, Lirong ; Liu, Yongsheng ; Xiao, Fangming. / Functional analysis of the seven in absentia ubiquitin ligase family in tomato. In: Plant Cell and Environment. 2018 ; Vol. 41, No. 3. pp. 689-703.
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