Functional analysis of free methionine-R-sulfoxide reductase from saccharomyces cerevisiae

Dung Tien Le, Byung Cheon Lee, Stefano M. Marino, Yan Zhang, Dmitri Fomenko, Alaattin Kaya, Elise Hacioglu, Geun Hee Kwak, Ahmet Koc, Hwa Young Kim, Vadim N. Gladyshev

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Abstract

Methionine sulfoxide reductases (Msrs) are oxidoreductases that catalyze thiol-dependent reduction of oxidized methionines. MsrA and MsrB are the best known Msrs that repair methionine S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO) residues in proteins, respectively. In addition, an Escherichia coli enzyme specific for free Met-R-SO, designated fRMsr, was recently discovered. In this work, we carried out comparative genomic and experimental analyses to examine occurrence, evolution, and function of fRMsr. This protein is present in single copies and two mutually exclusive subtypes in about half of prokaryotes and unicellular eukaryotes but is missing in higher plants and animals. A Saccharomyces cerevisiae fRMsr homolog was found to reduce free Met-R-SO but not free Met-S-SO or dabsyl-Met-R-SO. fRMsr was responsible for growth of yeast cells on Met-R-SO, and the double fRMsr/MsrA mutant could not grow on a mixture of methionine sulfoxides. However, in the presence of methionine, even the triple fRMsr/MsrA/MsrB mutant was viable. In addition, fRMsr deletion strain showed an increased sensitivity to oxidative stress and a decreased life span, whereas overexpression of fRMsr conferred higher resistance to oxidants. Molecular modeling and cysteine residue targeting by thioredoxin pointed to Cys101 as catalytic and Cys125 as resolving residues in yeast fRMsr. These residues as well as a third Cys, resolving Cys91, clustered in the structure, and each was required for the catalytic activity of the enzyme. The data show that fRMsr is the main enzyme responsible for the reduction of free Met-R-SO in S. cerevisiae.

Original languageEnglish (US)
Pages (from-to)4354-4364
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number7
DOIs
StatePublished - Feb 13 2009

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sulfoxide
Methionine Sulfoxide Reductases
Functional analysis
Methionine
Yeast
Saccharomyces cerevisiae
Repair
Enzymes
Yeasts
methionine sulfoxide
Thioredoxins
Oxidative stress
Molecular modeling
Eukaryota
Sulfhydryl Compounds
Oxidants
Escherichia coli
Cysteine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functional analysis of free methionine-R-sulfoxide reductase from saccharomyces cerevisiae. / Le, Dung Tien; Lee, Byung Cheon; Marino, Stefano M.; Zhang, Yan; Fomenko, Dmitri; Kaya, Alaattin; Hacioglu, Elise; Kwak, Geun Hee; Koc, Ahmet; Kim, Hwa Young; Gladyshev, Vadim N.

In: Journal of Biological Chemistry, Vol. 284, No. 7, 13.02.2009, p. 4354-4364.

Research output: Contribution to journalArticle

Le, DT, Lee, BC, Marino, SM, Zhang, Y, Fomenko, D, Kaya, A, Hacioglu, E, Kwak, GH, Koc, A, Kim, HY & Gladyshev, VN 2009, 'Functional analysis of free methionine-R-sulfoxide reductase from saccharomyces cerevisiae', Journal of Biological Chemistry, vol. 284, no. 7, pp. 4354-4364. https://doi.org/10.1074/jbc.M805891200
Le, Dung Tien ; Lee, Byung Cheon ; Marino, Stefano M. ; Zhang, Yan ; Fomenko, Dmitri ; Kaya, Alaattin ; Hacioglu, Elise ; Kwak, Geun Hee ; Koc, Ahmet ; Kim, Hwa Young ; Gladyshev, Vadim N. / Functional analysis of free methionine-R-sulfoxide reductase from saccharomyces cerevisiae. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 7. pp. 4354-4364.
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abstract = "Methionine sulfoxide reductases (Msrs) are oxidoreductases that catalyze thiol-dependent reduction of oxidized methionines. MsrA and MsrB are the best known Msrs that repair methionine S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO) residues in proteins, respectively. In addition, an Escherichia coli enzyme specific for free Met-R-SO, designated fRMsr, was recently discovered. In this work, we carried out comparative genomic and experimental analyses to examine occurrence, evolution, and function of fRMsr. This protein is present in single copies and two mutually exclusive subtypes in about half of prokaryotes and unicellular eukaryotes but is missing in higher plants and animals. A Saccharomyces cerevisiae fRMsr homolog was found to reduce free Met-R-SO but not free Met-S-SO or dabsyl-Met-R-SO. fRMsr was responsible for growth of yeast cells on Met-R-SO, and the double fRMsr/MsrA mutant could not grow on a mixture of methionine sulfoxides. However, in the presence of methionine, even the triple fRMsr/MsrA/MsrB mutant was viable. In addition, fRMsr deletion strain showed an increased sensitivity to oxidative stress and a decreased life span, whereas overexpression of fRMsr conferred higher resistance to oxidants. Molecular modeling and cysteine residue targeting by thioredoxin pointed to Cys101 as catalytic and Cys125 as resolving residues in yeast fRMsr. These residues as well as a third Cys, resolving Cys91, clustered in the structure, and each was required for the catalytic activity of the enzyme. The data show that fRMsr is the main enzyme responsible for the reduction of free Met-R-SO in S. cerevisiae.",
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AU - Zhang, Yan

AU - Fomenko, Dmitri

AU - Kaya, Alaattin

AU - Hacioglu, Elise

AU - Kwak, Geun Hee

AU - Koc, Ahmet

AU - Kim, Hwa Young

AU - Gladyshev, Vadim N.

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