Functional activity of commercial prebiotics

J. Huebner, R. L. Wehling, Robert W Hutkins

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

This work established a quantitative score to describe the extent to which prebiotics (fructooligosaccharides, inulin, and galactooligosaccharides) support selective growth of lactobacilli and bifidobacteria. The prebiotic activity assay was based on the change in cell biomass after 24 h of growth of the probiotic strain on 1% prebiotic or 1% glucose relative to the change in cell biomass of a mixture of enteric strains grown under the same conditions. From the biomass data, a prebiotic activity score was calculated for five lactobacilli and five bifidobacteria. In general, the scores were dependent on the probiotic bacterial strain tested and the type of prebiotic carbohydrate utilized. The highest score was obtained for Lactobacillus paracasei 1195 grown on inulin (1.17), and the lowest score was for Bifidobacterium bifidum NCI grown on galactooligosaccharides (-1.24). Results reported here provide a basis for evaluating and optimizing combinations of probiotics and prebiotics for applications as synbiotics.

Original languageEnglish (US)
Pages (from-to)770-775
Number of pages6
JournalInternational Dairy Journal
Volume17
Issue number7
DOIs
StatePublished - Jul 1 2007

Fingerprint

Prebiotics
prebiotics
Probiotics
Biomass
probiotics
galactooligosaccharides
Bifidobacterium
Inulin
inulin
Lactobacillus
biomass
Synbiotics
Bifidobacterium bifidum
Lactobacillus paracasei
fructooligosaccharides
Growth
dietary supplements
Carbohydrates
cells
carbohydrates

Keywords

  • Fructooligosaccharides
  • Galactooligosaccharides
  • Inulin
  • Prebiotics
  • Probiotics

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Functional activity of commercial prebiotics. / Huebner, J.; Wehling, R. L.; Hutkins, Robert W.

In: International Dairy Journal, Vol. 17, No. 7, 01.07.2007, p. 770-775.

Research output: Contribution to journalArticle

Huebner, J. ; Wehling, R. L. ; Hutkins, Robert W. / Functional activity of commercial prebiotics. In: International Dairy Journal. 2007 ; Vol. 17, No. 7. pp. 770-775.
@article{46a8d663fba0465a873fa0adc6b8a1dd,
title = "Functional activity of commercial prebiotics",
abstract = "This work established a quantitative score to describe the extent to which prebiotics (fructooligosaccharides, inulin, and galactooligosaccharides) support selective growth of lactobacilli and bifidobacteria. The prebiotic activity assay was based on the change in cell biomass after 24 h of growth of the probiotic strain on 1{\%} prebiotic or 1{\%} glucose relative to the change in cell biomass of a mixture of enteric strains grown under the same conditions. From the biomass data, a prebiotic activity score was calculated for five lactobacilli and five bifidobacteria. In general, the scores were dependent on the probiotic bacterial strain tested and the type of prebiotic carbohydrate utilized. The highest score was obtained for Lactobacillus paracasei 1195 grown on inulin (1.17), and the lowest score was for Bifidobacterium bifidum NCI grown on galactooligosaccharides (-1.24). Results reported here provide a basis for evaluating and optimizing combinations of probiotics and prebiotics for applications as synbiotics.",
keywords = "Fructooligosaccharides, Galactooligosaccharides, Inulin, Prebiotics, Probiotics",
author = "J. Huebner and Wehling, {R. L.} and Hutkins, {Robert W}",
year = "2007",
month = "7",
day = "1",
doi = "10.1016/j.idairyj.2006.10.006",
language = "English (US)",
volume = "17",
pages = "770--775",
journal = "International Dairy Journal",
issn = "0958-6946",
publisher = "Elsevier BV",
number = "7",

}

TY - JOUR

T1 - Functional activity of commercial prebiotics

AU - Huebner, J.

AU - Wehling, R. L.

AU - Hutkins, Robert W

PY - 2007/7/1

Y1 - 2007/7/1

N2 - This work established a quantitative score to describe the extent to which prebiotics (fructooligosaccharides, inulin, and galactooligosaccharides) support selective growth of lactobacilli and bifidobacteria. The prebiotic activity assay was based on the change in cell biomass after 24 h of growth of the probiotic strain on 1% prebiotic or 1% glucose relative to the change in cell biomass of a mixture of enteric strains grown under the same conditions. From the biomass data, a prebiotic activity score was calculated for five lactobacilli and five bifidobacteria. In general, the scores were dependent on the probiotic bacterial strain tested and the type of prebiotic carbohydrate utilized. The highest score was obtained for Lactobacillus paracasei 1195 grown on inulin (1.17), and the lowest score was for Bifidobacterium bifidum NCI grown on galactooligosaccharides (-1.24). Results reported here provide a basis for evaluating and optimizing combinations of probiotics and prebiotics for applications as synbiotics.

AB - This work established a quantitative score to describe the extent to which prebiotics (fructooligosaccharides, inulin, and galactooligosaccharides) support selective growth of lactobacilli and bifidobacteria. The prebiotic activity assay was based on the change in cell biomass after 24 h of growth of the probiotic strain on 1% prebiotic or 1% glucose relative to the change in cell biomass of a mixture of enteric strains grown under the same conditions. From the biomass data, a prebiotic activity score was calculated for five lactobacilli and five bifidobacteria. In general, the scores were dependent on the probiotic bacterial strain tested and the type of prebiotic carbohydrate utilized. The highest score was obtained for Lactobacillus paracasei 1195 grown on inulin (1.17), and the lowest score was for Bifidobacterium bifidum NCI grown on galactooligosaccharides (-1.24). Results reported here provide a basis for evaluating and optimizing combinations of probiotics and prebiotics for applications as synbiotics.

KW - Fructooligosaccharides

KW - Galactooligosaccharides

KW - Inulin

KW - Prebiotics

KW - Probiotics

UR - http://www.scopus.com/inward/record.url?scp=33947681294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947681294&partnerID=8YFLogxK

U2 - 10.1016/j.idairyj.2006.10.006

DO - 10.1016/j.idairyj.2006.10.006

M3 - Article

VL - 17

SP - 770

EP - 775

JO - International Dairy Journal

JF - International Dairy Journal

SN - 0958-6946

IS - 7

ER -