Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways

T. Asai, J. M. Stone, J. E. Heard, Y. Kovtun, P. Yorgey, J. Sheen, F. M. Ausubel

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

We have established an Arabidopsis protoplast model system to study plant cell death signaling. The fungal toxin fumonisin B1 (FB1) induces apoptosis-like programmed cell death (PCD) in wild-type protoplasts. FB1, however, only marginally affects the viability of protoplasts isolated from transgenic NahG plants, in which salicylic acid (SA) is metabolically degraded; from pad4-1 mutant plants, in which an SA amplification mechanism is thought to be impaired; or from jar1-1 or etr1-1 mutant plants, which are insensitive to jasmonate (JA) or ethylene (ET), respectively. FB1 susceptibility of wild-type protoplasts decreases in the dark, as does the cellular content of phenylalanine ammonia-lyase, a light-inducible enzyme involved in SA biosynthesis. Interestingly, however, FB1-induced PCD does not require the SA signal transmitter NPR1, given that npr1-1 protoplasts display wild-type FB1 susceptibility. Arabidopsis cpr1-1, cpr6-1, and acd2-2 protoplasts, in which the SA signaling pathway is constitutively activated, exhibit increased susceptibility to FB1. The cpr6-1 and acd2-2 mutants also constitutively express the JA and ET signaling pathways, but only the acd2-2 protoplasts undergo PCD in the absence of FB1. These results demonstrate that FB1 killing of Arabidopsis is light dependent and requires SA-, JA-, and ET-mediated signaling pathways as well as one or more unidentified factors activated by FB1 and the acd2-2 mutation.

Original languageEnglish (US)
Pages (from-to)1823-1835
Number of pages13
JournalPlant Cell
Volume12
Issue number10
DOIs
StatePublished - Nov 22 2000

Fingerprint

salicylates
fumonisin B1
Protoplasts
Salicylates
Cell death
Arabidopsis
protoplasts
cell death
ethylene
Cell Death
Salicylic Acid
salicylic acid
apoptosis
mutants
Phenylalanine Ammonia-Lyase
jasmonic acid
Light
Mycotoxins
Genetically Modified Plants
Biosynthesis

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. / Asai, T.; Stone, J. M.; Heard, J. E.; Kovtun, Y.; Yorgey, P.; Sheen, J.; Ausubel, F. M.

In: Plant Cell, Vol. 12, No. 10, 22.11.2000, p. 1823-1835.

Research output: Contribution to journalArticle

Asai, T. ; Stone, J. M. ; Heard, J. E. ; Kovtun, Y. ; Yorgey, P. ; Sheen, J. ; Ausubel, F. M. / Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. In: Plant Cell. 2000 ; Vol. 12, No. 10. pp. 1823-1835.
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