Fum3p, a 2-Ketoglutarate-Dependent Dioxygenase Required for C-5 Hydroxylation of Fumonisins in Fusarium verticillioides

Yousong Ding, Ravi S. Bojja, Liangcheng Du

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Fumonisins are polyketide-derived mycotoxins produced by several agriculturally important Fusarium species. The B series fumonisins, FB 1, FB2, FB3, and FB4, are fumonisins produced by wild-type Fusarium verticillioides strains, differing in the number and location of hydroxyl groups attached to the carbon backbone. We characterized the protein encoded by FUM3, a gene in the fumonisin biosynthetic gene cluster. The 33-kDa FUM3 protein (Fum3p) was heterologously expressed and purified from Saccharomyces cerevisiae. Yeast cells expressing the Fum3p converted FB3 to FB1, indicating that Fum3p catalyzes the C-5 hydroxylation of fumonisins. This result was verified by assaying the activity of Fum3p purified from yeast cells. The C-5 hydroxylase activity of purified Fum3p required 2-ketoglutarate, Fe2+, ascorbic acid, and catalase, all of which are required for 2-ketoglutarate-dependent dioxygenases. The protein also contains two His motifs that are highly conserved in this family of dioxygenases. Thus, Fum3p is a 2-ketoglutarate-dependent dioxygenase required for the addition of the C-5 hydroxyl group of fumonisins.

Original languageEnglish (US)
Pages (from-to)1931-1934
Number of pages4
JournalApplied and environmental microbiology
Volume70
Issue number4
DOIs
StatePublished - Apr 1 2004

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Fumonisins
Dioxygenases
fumonisins
Fusarium
hydroxylation
Hydroxylation
protein
Proteins
proteins
Hydroxyl Radical
yeast
Polyketides
Fungal Proteins
Mycotoxins
Multigene Family
yeasts
Mixed Function Oxygenases
Protein C
polyketides
Catalase

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Fum3p, a 2-Ketoglutarate-Dependent Dioxygenase Required for C-5 Hydroxylation of Fumonisins in Fusarium verticillioides. / Ding, Yousong; Bojja, Ravi S.; Du, Liangcheng.

In: Applied and environmental microbiology, Vol. 70, No. 4, 01.04.2004, p. 1931-1934.

Research output: Contribution to journalArticle

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