Frog vasoactive intestinal polypeptide and galanin: Primary structures and effects on pituitary adenylate cyclase

Nicolas Chartrel, Yunxia Wang, Alain Fournier, Hubert Vaudry, J. Michael Conlon

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Vasoactive intestinal polypeptide (VIP) and galanin were isolated in pure form from the stomach of the European green frog, Rana ridibunda. Frog VIP is identical to the previously characterized VIP from chicken and alligator. The primary structure of frog galanin contains only two amino acid substitutions (asparagine for histidine at position 23 and histidine for tyrosine at position 26) compared with porcine galanin. The data indicate that evolutionary pressure to conserve the amino acid sequence of both peptides during the evolution of amphibia to mammals has been strong. Synthetic frog VIP produced a dose-dependent increase in cAMP concentration in frog anterior pituitary fragments. The potency of the peptide (ED50 = 1.2 × 10-6 M; mean ± SE; n = 8) was comparable to that of porcine VIP (EC50 = 1.3 × 10-6 M), but was appro×imately 10-fold less than that of frog pituitary adenylate cyclase-activating polypeptide [PACAP-(1-38); ED50 = 1.1 × 10-7 M] in the same system. The increases in cAMP concentrations produced by ma×imal doses of PACAP (10-5 M) and VIP (10-5 M) were not additive. The data suggest that the effects of both peptides are mediated through a common PACAP-preferring receptor that is pharmacologically different from the mammalian PACAP type I receptor. Synthetic frog galanin also produced a dose-dependent increase in the concentration of cAMP in isolated frog anterior pituitary fragments (ED50 = 9.3 × 10-8 M) consistent with a possible role for the peptide as a hypophysiotropic factor in amphibians.

Original languageEnglish (US)
Pages (from-to)3079-3086
Number of pages8
JournalEndocrinology
Volume136
Issue number7
DOIs
StatePublished - Jul 1995

Fingerprint

Galanin
Vasoactive Intestinal Peptide
Adenylyl Cyclases
Anura
Pituitary Adenylate Cyclase-Activating Polypeptide
Peptides
Amphibians
Histidine
Rana clamitans
Swine
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Rana ridibunda
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Alligators and Crocodiles
Asparagine
Amino Acid Substitution
Tyrosine
Mammals
Amino Acid Sequence
Chickens

ASJC Scopus subject areas

  • Endocrinology

Cite this

Frog vasoactive intestinal polypeptide and galanin : Primary structures and effects on pituitary adenylate cyclase. / Chartrel, Nicolas; Wang, Yunxia; Fournier, Alain; Vaudry, Hubert; Conlon, J. Michael.

In: Endocrinology, Vol. 136, No. 7, 07.1995, p. 3079-3086.

Research output: Contribution to journalArticle

Chartrel, Nicolas ; Wang, Yunxia ; Fournier, Alain ; Vaudry, Hubert ; Conlon, J. Michael. / Frog vasoactive intestinal polypeptide and galanin : Primary structures and effects on pituitary adenylate cyclase. In: Endocrinology. 1995 ; Vol. 136, No. 7. pp. 3079-3086.
@article{9d9f7ad6cd6546729ec2162e886b4170,
title = "Frog vasoactive intestinal polypeptide and galanin: Primary structures and effects on pituitary adenylate cyclase",
abstract = "Vasoactive intestinal polypeptide (VIP) and galanin were isolated in pure form from the stomach of the European green frog, Rana ridibunda. Frog VIP is identical to the previously characterized VIP from chicken and alligator. The primary structure of frog galanin contains only two amino acid substitutions (asparagine for histidine at position 23 and histidine for tyrosine at position 26) compared with porcine galanin. The data indicate that evolutionary pressure to conserve the amino acid sequence of both peptides during the evolution of amphibia to mammals has been strong. Synthetic frog VIP produced a dose-dependent increase in cAMP concentration in frog anterior pituitary fragments. The potency of the peptide (ED50 = 1.2 × 10-6 M; mean ± SE; n = 8) was comparable to that of porcine VIP (EC50 = 1.3 × 10-6 M), but was appro×imately 10-fold less than that of frog pituitary adenylate cyclase-activating polypeptide [PACAP-(1-38); ED50 = 1.1 × 10-7 M] in the same system. The increases in cAMP concentrations produced by ma×imal doses of PACAP (10-5 M) and VIP (10-5 M) were not additive. The data suggest that the effects of both peptides are mediated through a common PACAP-preferring receptor that is pharmacologically different from the mammalian PACAP type I receptor. Synthetic frog galanin also produced a dose-dependent increase in the concentration of cAMP in isolated frog anterior pituitary fragments (ED50 = 9.3 × 10-8 M) consistent with a possible role for the peptide as a hypophysiotropic factor in amphibians.",
author = "Nicolas Chartrel and Yunxia Wang and Alain Fournier and Hubert Vaudry and Conlon, {J. Michael}",
year = "1995",
month = "7",
doi = "10.1210/endo.136.7.7540547",
language = "English (US)",
volume = "136",
pages = "3079--3086",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "7",

}

TY - JOUR

T1 - Frog vasoactive intestinal polypeptide and galanin

T2 - Primary structures and effects on pituitary adenylate cyclase

AU - Chartrel, Nicolas

AU - Wang, Yunxia

AU - Fournier, Alain

AU - Vaudry, Hubert

AU - Conlon, J. Michael

PY - 1995/7

Y1 - 1995/7

N2 - Vasoactive intestinal polypeptide (VIP) and galanin were isolated in pure form from the stomach of the European green frog, Rana ridibunda. Frog VIP is identical to the previously characterized VIP from chicken and alligator. The primary structure of frog galanin contains only two amino acid substitutions (asparagine for histidine at position 23 and histidine for tyrosine at position 26) compared with porcine galanin. The data indicate that evolutionary pressure to conserve the amino acid sequence of both peptides during the evolution of amphibia to mammals has been strong. Synthetic frog VIP produced a dose-dependent increase in cAMP concentration in frog anterior pituitary fragments. The potency of the peptide (ED50 = 1.2 × 10-6 M; mean ± SE; n = 8) was comparable to that of porcine VIP (EC50 = 1.3 × 10-6 M), but was appro×imately 10-fold less than that of frog pituitary adenylate cyclase-activating polypeptide [PACAP-(1-38); ED50 = 1.1 × 10-7 M] in the same system. The increases in cAMP concentrations produced by ma×imal doses of PACAP (10-5 M) and VIP (10-5 M) were not additive. The data suggest that the effects of both peptides are mediated through a common PACAP-preferring receptor that is pharmacologically different from the mammalian PACAP type I receptor. Synthetic frog galanin also produced a dose-dependent increase in the concentration of cAMP in isolated frog anterior pituitary fragments (ED50 = 9.3 × 10-8 M) consistent with a possible role for the peptide as a hypophysiotropic factor in amphibians.

AB - Vasoactive intestinal polypeptide (VIP) and galanin were isolated in pure form from the stomach of the European green frog, Rana ridibunda. Frog VIP is identical to the previously characterized VIP from chicken and alligator. The primary structure of frog galanin contains only two amino acid substitutions (asparagine for histidine at position 23 and histidine for tyrosine at position 26) compared with porcine galanin. The data indicate that evolutionary pressure to conserve the amino acid sequence of both peptides during the evolution of amphibia to mammals has been strong. Synthetic frog VIP produced a dose-dependent increase in cAMP concentration in frog anterior pituitary fragments. The potency of the peptide (ED50 = 1.2 × 10-6 M; mean ± SE; n = 8) was comparable to that of porcine VIP (EC50 = 1.3 × 10-6 M), but was appro×imately 10-fold less than that of frog pituitary adenylate cyclase-activating polypeptide [PACAP-(1-38); ED50 = 1.1 × 10-7 M] in the same system. The increases in cAMP concentrations produced by ma×imal doses of PACAP (10-5 M) and VIP (10-5 M) were not additive. The data suggest that the effects of both peptides are mediated through a common PACAP-preferring receptor that is pharmacologically different from the mammalian PACAP type I receptor. Synthetic frog galanin also produced a dose-dependent increase in the concentration of cAMP in isolated frog anterior pituitary fragments (ED50 = 9.3 × 10-8 M) consistent with a possible role for the peptide as a hypophysiotropic factor in amphibians.

UR - http://www.scopus.com/inward/record.url?scp=0029065451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029065451&partnerID=8YFLogxK

U2 - 10.1210/endo.136.7.7540547

DO - 10.1210/endo.136.7.7540547

M3 - Article

C2 - 7540547

AN - SCOPUS:0029065451

VL - 136

SP - 3079

EP - 3086

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 7

ER -