Frizzled-4 expression during chick kidney development

Michael R. Stark, Mahendra S. Rao, Gary C. Schoenwolf, Guizhi Yang, Danielle Smith, Teri J. Mauch

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Wnts have been implicated in metanephric kidney development. To determine whether Frizzleds, the genes that encode Wnt receptors, are present at early stages of nephrogenesis, we examined the expression of several recently identified Frizzled genes in the chick by in situ hybridization. Here we report the cloning and characterization of chick Frizzled-4 (cFz-4), which we found to be expressed in the developing chick kidney. cFz-4 was first expressed in the pronephros caudal to the third somite at Hamburger and Hamilton stage 10. Its expression increased with maturation, becoming restricted to the newly induced glomeruli and tubules in the mesonephros and metanephros. Within the metanephros, cFz-4 and Wnt-4 expression patterns were similar, whereas Wnt-11 was expressed solely in the tips of the branching ureteric bud. cFz-4 expression was compared with that of known kidney markers. It preceded that of Lmx-1, but was similarly restricted to developing glomeruli and tubules. In contrast, Pax-2 expression and Lim 1/2 antibody labeling occurred in intermediate mesoderm caudal to the fifth somite in the early pronephros, and each persisted in both the tubules and nephric ducts throughout further development. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalMechanisms of Development
Volume98
Issue number1-2
DOIs
StatePublished - Nov 1 2000

Fingerprint

Pronephros
Kidney
Somites
Wnt Receptors
Mesonephros
Mesoderm
Genes
In Situ Hybridization
Organism Cloning
Antibodies

Keywords

  • In situ hybridization
  • Lim 1/2
  • Lmx-1
  • Mesonephros
  • Metanephros
  • Pax-2
  • Pronephros
  • Wnt-11
  • Wnt-4

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Stark, M. R., Rao, M. S., Schoenwolf, G. C., Yang, G., Smith, D., & Mauch, T. J. (2000). Frizzled-4 expression during chick kidney development. Mechanisms of Development, 98(1-2), 121-125. https://doi.org/10.1016/S0925-4773(00)00440-8

Frizzled-4 expression during chick kidney development. / Stark, Michael R.; Rao, Mahendra S.; Schoenwolf, Gary C.; Yang, Guizhi; Smith, Danielle; Mauch, Teri J.

In: Mechanisms of Development, Vol. 98, No. 1-2, 01.11.2000, p. 121-125.

Research output: Contribution to journalArticle

Stark, MR, Rao, MS, Schoenwolf, GC, Yang, G, Smith, D & Mauch, TJ 2000, 'Frizzled-4 expression during chick kidney development', Mechanisms of Development, vol. 98, no. 1-2, pp. 121-125. https://doi.org/10.1016/S0925-4773(00)00440-8
Stark, Michael R. ; Rao, Mahendra S. ; Schoenwolf, Gary C. ; Yang, Guizhi ; Smith, Danielle ; Mauch, Teri J. / Frizzled-4 expression during chick kidney development. In: Mechanisms of Development. 2000 ; Vol. 98, No. 1-2. pp. 121-125.
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