Frequency sensitive mechanism in low-intensity ultrasound enhanced bioeffects

April D. Miller, Abdoulkadri Chama, Tobias M. Louw, Anuradha Subramanian, Hendrik J Viljoen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study presents two novel theoretical models to elucidate frequency sensitive nuclear mechanisms in low-intensity ultrasound enhanced bioeffects. In contrast to the typical 1.5 MHz pulsed ultrasound regime, our group previously experimentally confirmed that ultrasound stimulation of anchored chondrocytes at resonant frequency maximized gene expression of load inducible genes which are regulatory markers for cellular response to external stimuli. However, ERK phosphorylation displayed no frequency dependency, suggesting that the biochemical mechanisms involved in enhanced gene expression is downstream of ERK phosphorylation. To elucidate such underlying mechanisms, this study presents a theoretical model of an anchored cell, representing an in vitro chondrocyte, in an ultrasound field. The model results showed that the mechanical energy storage is maximized at the chondrocyte’s resonant frequency and the energy density in the nucleus is almost twice as high as in the cytoplasm. Next, a mechanochemical model was developed to link the mechanical stimulation of ultrasound and the increased mechanical energy density in the nucleus to the downstream targets of the ERK pathway. This study showed for the first time that ultrasound stimulation induces frequency dependent gene expression as a result of altered rates of transcription factors binding to chromatin.

Original languageEnglish (US)
Article numbere0181717
JournalPloS one
Volume12
Issue number8
DOIs
StatePublished - Aug 1 2017

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Chondrocytes
Ultrasonics
chondrocytes
Gene Expression
Theoretical Models
Phosphorylation
Gene expression
energy density
MAP Kinase Signaling System
gene expression
Regulator Genes
phosphorylation
Chromatin
Natural frequencies
Cytoplasm
Transcription Factors
biochemical mechanisms
chromatin
cytoplasm
transcription factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Frequency sensitive mechanism in low-intensity ultrasound enhanced bioeffects. / Miller, April D.; Chama, Abdoulkadri; Louw, Tobias M.; Subramanian, Anuradha; Viljoen, Hendrik J.

In: PloS one, Vol. 12, No. 8, e0181717, 01.08.2017.

Research output: Contribution to journalArticle

Miller, April D. ; Chama, Abdoulkadri ; Louw, Tobias M. ; Subramanian, Anuradha ; Viljoen, Hendrik J. / Frequency sensitive mechanism in low-intensity ultrasound enhanced bioeffects. In: PloS one. 2017 ; Vol. 12, No. 8.
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