Formate dehydrogenase in Arabidopsis thaliana: Overexpression and subcellular localization in leaves

Patricia L. Herman, Håkon Ramberg, Renee D. Baack, John Markwell, John C. Osterman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Formate dehydrogenase (FDH; EC 1.2.1.2) is a NAD-dependent enzyme that catalyzes the oxidation of formate to carbon dioxide in the mitochondria of higher plants. Sequence analyses and other preliminary experiments suggested that FDH might also be targeted to the chloroplasts of Arabidopsis thaliana and other plant species. In the present study, transgenic Arabidopsis and tobacco plants that overexpress Arabidopsis FDH were produced. The FDH specific activity in the leaf tissue of the transgenic plants increased an average of 4.5-fold for Arabidopsis and 31.5-fold for tobacco. Immunodetection and enzyme assays of intact chloroplasts fractionated from the leaves of transgenic tobacco plants suggested that Arabidopsis FDH is present in the chloroplast. Immunogold labeling of Arabidopsis and tobacco detected FDH in both the mitochondria and chloroplasts of the leaf cells. Enzyme assays that were performed to confirm the specificity of Arabidopsis FDH for the NAD cofactor suggested that NADPH was an inhibitor of NAD+ dependent formate oxidation.

Original languageEnglish (US)
Pages (from-to)1137-1145
Number of pages9
JournalPlant Science
Volume163
Issue number6
DOIs
StatePublished - Dec 1 2002

Fingerprint

Formate Dehydrogenases
formic acid
Tobacco
Arabidopsis
Arabidopsis thaliana
NAD
Mitochondria
Chloroplasts
NAD (coenzyme)
tobacco
chloroplasts
leaves
Assays
Enzymes
formates
Genetically Modified Plants
Oxidation
Enzyme Assays
Corrosion inhibitors
mitochondria

Keywords

  • Arabidopsis
  • Chloroplasts
  • Formate dehydrogenase
  • Mitochondria
  • NADPH
  • Tobacco

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Formate dehydrogenase in Arabidopsis thaliana : Overexpression and subcellular localization in leaves. / Herman, Patricia L.; Ramberg, Håkon; Baack, Renee D.; Markwell, John; Osterman, John C.

In: Plant Science, Vol. 163, No. 6, 01.12.2002, p. 1137-1145.

Research output: Contribution to journalArticle

Herman, Patricia L. ; Ramberg, Håkon ; Baack, Renee D. ; Markwell, John ; Osterman, John C. / Formate dehydrogenase in Arabidopsis thaliana : Overexpression and subcellular localization in leaves. In: Plant Science. 2002 ; Vol. 163, No. 6. pp. 1137-1145.
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