Formate dehydrogenase in Arabidopsis thaliana: Characterization and possible targeting to the chloroplast

Bradley J.S.C. Olson, Maryanne Skavdahl, Håkon Ramberg, John C. Osterman, John Markwell

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Formate dehydrogenase (E.C. 1.2.1.2) is a mitochondrial-localized NAD-requiring enzyme in green plants. The enzyme activity and corresponding mRNA in leaves of Arabidopsis thaliana are induced by treatment with one-carbon metabolites. The cDNA for the Arabidopsis formate dehydrogenase is similar to that of other plants except for the N-terminal region, which is predicted to target chloroplasts as well as mitochondria. The specific of activity of the enzyme in isolated chloroplasts suggests it is targeted to both mitochondria and chloroplasts in Arabidopsis. Formate dehydrogenase from Arabidopsis was partially purified and K(m) values for formate and NAD+ were determined to be 10 mM and 65 μM, respectively; the K1 for NADH was 17 μM. We conclude that formate dehydrogenase is normally present in Arabidopsis chloroplasts and that sensitivity to inhibition by NADH may play a role in whether cellular formate is assimilated or dissimilated. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalPlant Science
Volume159
Issue number2
DOIs
StatePublished - Nov 6 2000

Fingerprint

Formate Dehydrogenases
formic acid
Chloroplasts
Arabidopsis
NAD
Arabidopsis thaliana
chloroplasts
Mitochondria
formates
mitochondria
Enzymes
enzyme activity
Viridiplantae
Enzyme activity
Metabolites
Carbon
Complementary DNA
Messenger RNA
formate dehydrogenase
metabolites

Keywords

  • Arabidopsis thaliana
  • Chloroplast
  • Formate
  • Formate dehydrogenase
  • Mitochondria

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Formate dehydrogenase in Arabidopsis thaliana : Characterization and possible targeting to the chloroplast. / Olson, Bradley J.S.C.; Skavdahl, Maryanne; Ramberg, Håkon; Osterman, John C.; Markwell, John.

In: Plant Science, Vol. 159, No. 2, 06.11.2000, p. 205-212.

Research output: Contribution to journalArticle

Olson, Bradley J.S.C. ; Skavdahl, Maryanne ; Ramberg, Håkon ; Osterman, John C. ; Markwell, John. / Formate dehydrogenase in Arabidopsis thaliana : Characterization and possible targeting to the chloroplast. In: Plant Science. 2000 ; Vol. 159, No. 2. pp. 205-212.
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