Food Fish Identification from DNA Extraction Through Sequence Analysis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This experiment exposed 3rd and 4th y undergraduates and graduate students taking a course in advanced food analysis to DNA extraction, polymerase chain reaction (PCR), and DNA sequence analysis. Students provided their own fish sample, purchased from local grocery stores, and the class as a whole extracted DNA, which was then subjected to PCR, gel electrophoresis, and Sanger sequencing. Students retrieved their sequences and identified their fish using the NCBI BLAST nucleotide database. Slightly more than half of the samples yielded sequences identical or close to expected (based on the identification of the fish on the packaging); some other samples matched unanticipated fish or other organisms, due to an incomplete database, minor sequencing errors, or laboratory contamination (human and fungal sequences); 1 canned tuna sample identified as hake could represent food fraud.

Original languageEnglish (US)
Pages (from-to)116-120
Number of pages5
JournalJournal of Food Science Education
Volume14
Issue number3
DOIs
StatePublished - Jul 1 2015

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Sequence Analysis
Fishes
sequence analysis
food
Food
students
DNA
Students
fish
polymerase chain reaction
canned fish
Databases
Tuna
grocery stores
Food Analysis
fraud
Fraud
sampling
Polymerase Chain Reaction
genetic test

ASJC Scopus subject areas

  • Food Science
  • Education

Cite this

Food Fish Identification from DNA Extraction Through Sequence Analysis. / Hallen-Adams, Heather E.

In: Journal of Food Science Education, Vol. 14, No. 3, 01.07.2015, p. 116-120.

Research output: Contribution to journalArticle

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