Follicular placenta of the viviparous fish, Heterandria formosa

II. Ultrastructure and development of the follicular epithelium

Bryon Grove, John P. Wourms

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Embryos of the viviparous poeciliid fish, Heterandria formosa, develop to term in the ovarian follicle where they undergo a 3,900% increase in embryonic dry weight. Maternal‐embryonic nutrient transfer occurs across a follicular placenta that is formed by close apposition of the embryonic surface (i.e., the entire body surface during early gestation and the pericardial amnionserosa during mid‐late gestation) to the follicular epithelium. To complement our recent study of the embryonic component of the follicular placenta, we now describe the development and fine structure of the maternal component of the follicular placenta. Transmission electron microscopy reveals that the ultrastructure of the egg envelope and the follicular epithelium that invests vitellogenic oocytes is typical of that described for teleosts. The egg envelope is a dense matrix, penetrated by microvilli of the oocyte. The follicular epithelium consists of a single layer of cuboidal cells that lack apical microvilli, basal surface specializations, and junctional complexes. Follicle cells investing the youngest embryonic stage examined (Tavolga's and Rugh's stage 5–7 for Xiphophorus maculatus) also lack apical microvilli and basal specializations, but possess junctional complexes. In contrast, follicle cells that invest embryos at stage 10 and later display ultrastructural features characteristic of transporting epithelial cells. Apical microvilli and surface invaginations are present. The basal surface is extensively folded. Apical and basal coated pits are present. The cytoplasm contains a rough endoplasmic reticulum, Golgi complexes, and dense staining vesicles that appear to be lysosomes. The presence of numerous apically located electron‐lucent vesicles that appear to be derived from the apical surface further suggests that these follicle cells may absorb and process follicular fluid. The egg envelope, which remains intact throughout gestation and lacks perforations, becomes progressively thinner and less dense as gestation proceeds. We postulate that these ultrastructural features, which are not present in the follicles of the lecithotrophic poeciliid, Poecilia reticulata, are specializations for maternal‐embryonic nutrient transfer and that the egg envelope, follicular epithelium, and underlying capillary network form the maternal component of the follicular placenta. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)167-184
Number of pages18
JournalJournal of Morphology
Volume220
Issue number2
DOIs
StatePublished - Jan 1 1994

Fingerprint

follicular development
Microvilli
microvilli
Taiwan
placenta
Placenta
Ovum
ultrastructure
Fishes
epithelium
Epithelium
pregnancy
Pregnancy
fish
Oocytes
embryo (animal)
oocytes
Embryonic Structures
Mothers
Cyprinodontiformes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

Follicular placenta of the viviparous fish, Heterandria formosa : II. Ultrastructure and development of the follicular epithelium. / Grove, Bryon; Wourms, John P.

In: Journal of Morphology, Vol. 220, No. 2, 01.01.1994, p. 167-184.

Research output: Contribution to journalArticle

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