Folate analysis by capillary electrophoresis

N. D. Bills, Steven Heye Hinrichs, F. Perini, K. P. Mountjoy

Research output: Contribution to journalArticle

Abstract

Folate congeners differing in polyglutamate chain length, oxidation state of the pteridine ring and in 5' and 10' substituents were resolved and quantified using capillary electrophoresis (CE). Tetrahydrofolate, folinic acid (leucovorin) and folic acid monomers were resolved using an uncoated silica capillary. Resolution of oxidized folates with glutamate chain lengths of 1, 3, 5, and 7 and methotrexate triglutamate was achieved with a DB17 capillary column. High resolution (100,000 theoretical plates) and short run times (<20 min) with adequate sensitivity (20 pg/peak) make CE ideal for resolving the multiplicity of folate species found in complex biologic matrices. CE should be useful in studies of folate nutriture and food folate bioavailability as well as in studies of folate and antifolate metabolism in cancer, rheumatoid arthritis and other contexts where quick and sensitive measurement of individual folate species would yield useful information. (Supported by Grant 95-80, University of Nebraska Medical Center).

Original languageEnglish (US)
Pages (from-to)A801
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

Fingerprint

Capillary electrophoresis
capillary electrophoresis
Capillary Electrophoresis
Folic Acid
folic acid
Leucovorin
Chain length
pteridines
Pteridines
Polyglutamic Acid
Folic Acid Antagonists
methotrexate
rheumatoid arthritis
Metabolism
Silicon Dioxide
glutamates
silica
Biological Availability
bioavailability
Glutamic Acid

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Bills, N. D., Hinrichs, S. H., Perini, F., & Mountjoy, K. P. (1996). Folate analysis by capillary electrophoresis. FASEB Journal, 10(3), A801.

Folate analysis by capillary electrophoresis. / Bills, N. D.; Hinrichs, Steven Heye; Perini, F.; Mountjoy, K. P.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996, p. A801.

Research output: Contribution to journalArticle

Bills, ND, Hinrichs, SH, Perini, F & Mountjoy, KP 1996, 'Folate analysis by capillary electrophoresis', FASEB Journal, vol. 10, no. 3, pp. A801.
Bills ND, Hinrichs SH, Perini F, Mountjoy KP. Folate analysis by capillary electrophoresis. FASEB Journal. 1996 Dec 1;10(3):A801.
Bills, N. D. ; Hinrichs, Steven Heye ; Perini, F. ; Mountjoy, K. P. / Folate analysis by capillary electrophoresis. In: FASEB Journal. 1996 ; Vol. 10, No. 3. pp. A801.
@article{1e7162bdb7a945988e6a0f482d9c4922,
title = "Folate analysis by capillary electrophoresis",
abstract = "Folate congeners differing in polyglutamate chain length, oxidation state of the pteridine ring and in 5' and 10' substituents were resolved and quantified using capillary electrophoresis (CE). Tetrahydrofolate, folinic acid (leucovorin) and folic acid monomers were resolved using an uncoated silica capillary. Resolution of oxidized folates with glutamate chain lengths of 1, 3, 5, and 7 and methotrexate triglutamate was achieved with a DB17 capillary column. High resolution (100,000 theoretical plates) and short run times (<20 min) with adequate sensitivity (20 pg/peak) make CE ideal for resolving the multiplicity of folate species found in complex biologic matrices. CE should be useful in studies of folate nutriture and food folate bioavailability as well as in studies of folate and antifolate metabolism in cancer, rheumatoid arthritis and other contexts where quick and sensitive measurement of individual folate species would yield useful information. (Supported by Grant 95-80, University of Nebraska Medical Center).",
author = "Bills, {N. D.} and Hinrichs, {Steven Heye} and F. Perini and Mountjoy, {K. P.}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
pages = "A801",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Folate analysis by capillary electrophoresis

AU - Bills, N. D.

AU - Hinrichs, Steven Heye

AU - Perini, F.

AU - Mountjoy, K. P.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Folate congeners differing in polyglutamate chain length, oxidation state of the pteridine ring and in 5' and 10' substituents were resolved and quantified using capillary electrophoresis (CE). Tetrahydrofolate, folinic acid (leucovorin) and folic acid monomers were resolved using an uncoated silica capillary. Resolution of oxidized folates with glutamate chain lengths of 1, 3, 5, and 7 and methotrexate triglutamate was achieved with a DB17 capillary column. High resolution (100,000 theoretical plates) and short run times (<20 min) with adequate sensitivity (20 pg/peak) make CE ideal for resolving the multiplicity of folate species found in complex biologic matrices. CE should be useful in studies of folate nutriture and food folate bioavailability as well as in studies of folate and antifolate metabolism in cancer, rheumatoid arthritis and other contexts where quick and sensitive measurement of individual folate species would yield useful information. (Supported by Grant 95-80, University of Nebraska Medical Center).

AB - Folate congeners differing in polyglutamate chain length, oxidation state of the pteridine ring and in 5' and 10' substituents were resolved and quantified using capillary electrophoresis (CE). Tetrahydrofolate, folinic acid (leucovorin) and folic acid monomers were resolved using an uncoated silica capillary. Resolution of oxidized folates with glutamate chain lengths of 1, 3, 5, and 7 and methotrexate triglutamate was achieved with a DB17 capillary column. High resolution (100,000 theoretical plates) and short run times (<20 min) with adequate sensitivity (20 pg/peak) make CE ideal for resolving the multiplicity of folate species found in complex biologic matrices. CE should be useful in studies of folate nutriture and food folate bioavailability as well as in studies of folate and antifolate metabolism in cancer, rheumatoid arthritis and other contexts where quick and sensitive measurement of individual folate species would yield useful information. (Supported by Grant 95-80, University of Nebraska Medical Center).

UR - http://www.scopus.com/inward/record.url?scp=33749302106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749302106&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749302106

VL - 10

SP - A801

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -