Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers

Mohammad Nahid Andalib, Jeong Soon Lee, Ligyeom Ha, Yuris Dzenis, Jung Y Lim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

While electrospun nanofibers have demonstrated the potential for novel tissue engineering scaffolds, very little is known about the molecular mechanism of how cells sense and adapt to nanofibers. Here, we revealed the role of focal adhesion kinase (FAK), one of the key molecular sensors in the focal adhesion complex, in regulating mesenchymal stem cell (MSC) shaping on nanofibers. We produced uniaxially aligned and randomly distributed nanofibers from poly(l-lactic acid) to have the same diameters (about 130 nm) and evaluated MSC behavior on these nanofibers comparing with that on flat PLLA control. C3H10T1/2 murine MSCs exhibited upregulations in FAK expression and phosphorylation (pY397) on nanofibrous cultures as assessed by immunoblotting, and this trend was even greater on aligned nanofibers. MSCs showed significantly elongated and well-spread morphologies on aligned and random nanofibers, respectively. In the presence of FAK silencing via small hairpin RNA (shRNA), cell elongation length in the aligned nanofiber direction (cell major axis length) was significantly decreased, while cells still showed preferred orientation along the aligned nanofibers. On random nanofibers, MSCs with FAK-shRNA showed impaired cell spreading resulting in smaller cell area and higher circularity. Our study provides new data on how MSCs shape their morphologies on aligned and random nanofibrous cultures potentially via FAK-mediated mechanism.

Original languageEnglish (US)
Pages (from-to)920-925
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume473
Issue number4
DOIs
StatePublished - May 13 2016

Fingerprint

Nanofibers
Focal Adhesion Protein-Tyrosine Kinases
Stem cells
Stem Cells
Mesenchymal Stromal Cells
Small Interfering RNA
Tissue Scaffolds
Phosphorylation
Focal Adhesions
Bioelectric potentials
Tissue Engineering
Tissue engineering
Immunoblotting
Elongation
Lactic Acid
Up-Regulation
Adhesion

Keywords

  • Aligned and random nanofibers
  • Cell alignment and spreading
  • Focal adhesion kinase
  • Mesenchymal stem cells
  • shRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers. / Andalib, Mohammad Nahid; Lee, Jeong Soon; Ha, Ligyeom; Dzenis, Yuris; Lim, Jung Y.

In: Biochemical and Biophysical Research Communications, Vol. 473, No. 4, 13.05.2016, p. 920-925.

Research output: Contribution to journalArticle

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