Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis

M. P. Nandakumar, Renu Nandakumar, B. Mattiasson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A flow injection system for the on-line detection of the intracellular enzyme lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorimetric detection unit. The system with a linearity of 0.1-5.4 U LDH ml-1 was applied for the detection of intracellular accumulation of LDH during Lactococcus lactis subsp. lactis cultivation.

Original languageEnglish (US)
Pages (from-to)1453-1457
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number18
DOIs
StatePublished - Oct 26 2000

Fingerprint

Flow Injection Analysis
L-Lactate Dehydrogenase
Fermentation
Online Systems
Lactococcus lactis
Disintegration
Coloring Agents
Injections
Enzymes
Dyes
Oxidoreductases

Keywords

  • Flow injection binding assay
  • Intracellular enzyme
  • Lactate dehydrogenase
  • On-line cell breakage
  • On-line monitoring

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis. / Nandakumar, M. P.; Nandakumar, Renu; Mattiasson, B.

In: Biotechnology Letters, Vol. 22, No. 18, 26.10.2000, p. 1453-1457.

Research output: Contribution to journalArticle

Nandakumar, M. P. ; Nandakumar, Renu ; Mattiasson, B. / Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis. In: Biotechnology Letters. 2000 ; Vol. 22, No. 18. pp. 1453-1457.
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