Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis

M. P. Nandakumar, R. Nandakumar, B. Mattiasson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A flow injection system for the on-line detection of the intracellular enzyme lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorimetric detection unit. The system with a linearity of 0.1-5.4 U LDH ml-1 was applied for the detection of intracellular accumulation of LDH during Lactococcus lactis subsp. lactis cultivation.

Original languageEnglish (US)
Pages (from-to)1453-1457
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number18
DOIs
Publication statusPublished - Oct 26 2000

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Keywords

  • Flow injection binding assay
  • Intracellular enzyme
  • Lactate dehydrogenase
  • On-line cell breakage
  • On-line monitoring

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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