Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients

Bhavana J. Dave, Semyon A. Risin, Margaret R. Spitz, Sen Pathak

Research output: Contribution to journalArticle

Abstract

Genetic predisposition to lung cancer was determined by observing nonrandom chromosomal alterations in peripheral blood lymphocytes (PBLs) of lung cancer patients. The histological distribution of the cases showed that chromosomes 7 and 9 were frequently altered in squamous cell lung carcinoma (SCLC) patients. We analyzed PBLs of 26 SCLC patients and 5 controls using fluorescent in situ hybridization (FISH) with whole chromosome painting probes of chromosomes 7 and 9 to further investigate the frequency of rearrangements in these chromosomes. Our results suggested that seeking nonrandom aberrations in larger numbers of cells using FISH strengthened our previous observation of mosaicism and involvement of specific chromosomes in lung cancer patients. On combining our previous data, aberrations in chromosome 7 (16 of 26 patients), chromosome 9 (14 of 26), and the present study, we could actually pinpoint more individuals with abnormalities of chromosome 7 (23 of 26) and chromosome 9 (21 of 26). Thus, analyzing more cells in PBLs and adding FISH analysis serve as useful adjuncts to our studies of nonrandom chromosomal aberrations and genetic mosaicism.

Original languageEnglish (US)
Pages (from-to)1187-1192
Number of pages6
JournalAnticancer Research
Volume16
Issue number3 A
StatePublished - May 1 1996

Fingerprint

Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 7
Lung Neoplasms
Lymphocytes
Mosaicism
Squamous Cell Carcinoma
Chromosomes
Chromosome Painting
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 21
Lung
Genetic Predisposition to Disease
Chromosome Aberrations
Cell Count
Observation

Keywords

  • Chromosome painting
  • Fluorescent in situ hybridization
  • Lung cancer
  • Mosaicism

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients. / Dave, Bhavana J.; Risin, Semyon A.; Spitz, Margaret R.; Pathak, Sen.

In: Anticancer Research, Vol. 16, No. 3 A, 01.05.1996, p. 1187-1192.

Research output: Contribution to journalArticle

Dave, BJ, Risin, SA, Spitz, MR & Pathak, S 1996, 'Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients', Anticancer Research, vol. 16, no. 3 A, pp. 1187-1192.
Dave, Bhavana J. ; Risin, Semyon A. ; Spitz, Margaret R. ; Pathak, Sen. / Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients. In: Anticancer Research. 1996 ; Vol. 16, No. 3 A. pp. 1187-1192.
@article{c911c58f3f45491ba92dfd83f85d9c1b,
title = "Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients",
abstract = "Genetic predisposition to lung cancer was determined by observing nonrandom chromosomal alterations in peripheral blood lymphocytes (PBLs) of lung cancer patients. The histological distribution of the cases showed that chromosomes 7 and 9 were frequently altered in squamous cell lung carcinoma (SCLC) patients. We analyzed PBLs of 26 SCLC patients and 5 controls using fluorescent in situ hybridization (FISH) with whole chromosome painting probes of chromosomes 7 and 9 to further investigate the frequency of rearrangements in these chromosomes. Our results suggested that seeking nonrandom aberrations in larger numbers of cells using FISH strengthened our previous observation of mosaicism and involvement of specific chromosomes in lung cancer patients. On combining our previous data, aberrations in chromosome 7 (16 of 26 patients), chromosome 9 (14 of 26), and the present study, we could actually pinpoint more individuals with abnormalities of chromosome 7 (23 of 26) and chromosome 9 (21 of 26). Thus, analyzing more cells in PBLs and adding FISH analysis serve as useful adjuncts to our studies of nonrandom chromosomal aberrations and genetic mosaicism.",
keywords = "Chromosome painting, Fluorescent in situ hybridization, Lung cancer, Mosaicism",
author = "Dave, {Bhavana J.} and Risin, {Semyon A.} and Spitz, {Margaret R.} and Sen Pathak",
year = "1996",
month = "5",
day = "1",
language = "English (US)",
volume = "16",
pages = "1187--1192",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3 A",

}

TY - JOUR

T1 - Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients

AU - Dave, Bhavana J.

AU - Risin, Semyon A.

AU - Spitz, Margaret R.

AU - Pathak, Sen

PY - 1996/5/1

Y1 - 1996/5/1

N2 - Genetic predisposition to lung cancer was determined by observing nonrandom chromosomal alterations in peripheral blood lymphocytes (PBLs) of lung cancer patients. The histological distribution of the cases showed that chromosomes 7 and 9 were frequently altered in squamous cell lung carcinoma (SCLC) patients. We analyzed PBLs of 26 SCLC patients and 5 controls using fluorescent in situ hybridization (FISH) with whole chromosome painting probes of chromosomes 7 and 9 to further investigate the frequency of rearrangements in these chromosomes. Our results suggested that seeking nonrandom aberrations in larger numbers of cells using FISH strengthened our previous observation of mosaicism and involvement of specific chromosomes in lung cancer patients. On combining our previous data, aberrations in chromosome 7 (16 of 26 patients), chromosome 9 (14 of 26), and the present study, we could actually pinpoint more individuals with abnormalities of chromosome 7 (23 of 26) and chromosome 9 (21 of 26). Thus, analyzing more cells in PBLs and adding FISH analysis serve as useful adjuncts to our studies of nonrandom chromosomal aberrations and genetic mosaicism.

AB - Genetic predisposition to lung cancer was determined by observing nonrandom chromosomal alterations in peripheral blood lymphocytes (PBLs) of lung cancer patients. The histological distribution of the cases showed that chromosomes 7 and 9 were frequently altered in squamous cell lung carcinoma (SCLC) patients. We analyzed PBLs of 26 SCLC patients and 5 controls using fluorescent in situ hybridization (FISH) with whole chromosome painting probes of chromosomes 7 and 9 to further investigate the frequency of rearrangements in these chromosomes. Our results suggested that seeking nonrandom aberrations in larger numbers of cells using FISH strengthened our previous observation of mosaicism and involvement of specific chromosomes in lung cancer patients. On combining our previous data, aberrations in chromosome 7 (16 of 26 patients), chromosome 9 (14 of 26), and the present study, we could actually pinpoint more individuals with abnormalities of chromosome 7 (23 of 26) and chromosome 9 (21 of 26). Thus, analyzing more cells in PBLs and adding FISH analysis serve as useful adjuncts to our studies of nonrandom chromosomal aberrations and genetic mosaicism.

KW - Chromosome painting

KW - Fluorescent in situ hybridization

KW - Lung cancer

KW - Mosaicism

UR - http://www.scopus.com/inward/record.url?scp=0029947921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029947921&partnerID=8YFLogxK

M3 - Article

C2 - 8702234

AN - SCOPUS:0029947921

VL - 16

SP - 1187

EP - 1192

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3 A

ER -