Fluid flow shear stress over podocytes is increased in the solitary kidney

Tarak Srivastava, Gianni E. Celsi, Mukut Sharma, Hongying Dai, Ellen T. McCarthy, Melanie Ruiz, Patricia A. Cudmore, Uri S. Alon, Ram Sharma, Virginia A. Savin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

BackgroundGlomerular hyperfiltration is emerging as the key risk factor for progression of chronic kidney disease (CKD). Podocytes are exposed to fluid flow shear stress (FFSS) caused by the flow of ultrafiltrate within Bowman's space. The mechanism of hyperfiltration-induced podocyte injury is not clear. We postulated that glomerular hyperfiltration in solitary kidney increases FFSS over podocytes.MethodsInfant Sprague-Dawley rats at 5 days of age and C57BL/6J 14-week-old adult mice underwent unilateral nephrectomy. Micropuncture and morphological studies were then performed on 20-and 60-day-old rats. FFSS over podocytes in uninephrectomized rats and mice was calculated using the recently published equation by Friedrich et al. which includes the variables-single nephron glomerular filtration rate (SNGFR), filtration fraction (f), glomerular tuft diameter (2RT) and width of Bowman's space (s).ResultsGlomerular hypertrophy was observed in uninephrectomized rats and mice. Uninephrectomized rats on Day 20 showed a 2.0-fold increase in SNGFR, 1.0-fold increase in 2R T and 2.1-fold increase in FFSS, and on Day 60 showed a 1.9-fold increase in SNGFR, 1.3-fold increase in 2RT and 1.5-fold increase in FFSS, at all values of modeled 's'. Similarly, uninephrectomized mice showed a 2-to 3-fold increase in FFSS at all values of modeled SNGFR.ConclusionsFFSS over podocytes is increased in solitary kidneys in both infant rats and adult mice. This increase is a consequence of increased SNGFR. We speculate that increased FFSS caused by reduced nephron number contributes to podocyte injury and promotes the progression of CKD.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalNephrology Dialysis Transplantation
Volume29
Issue number1
DOIs
StatePublished - Jan 27 2014
Externally publishedYes

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Podocytes
Nephrons
Glomerular Filtration Rate
Kidney
Chronic Renal Insufficiency
Wounds and Injuries
Nephrectomy
Punctures
Hypertrophy
Sprague Dawley Rats

Keywords

  • fluid flow shear stress
  • glomerular hyperfiltration
  • podocyte
  • solitary kidney

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Srivastava, T., Celsi, G. E., Sharma, M., Dai, H., McCarthy, E. T., Ruiz, M., ... Savin, V. A. (2014). Fluid flow shear stress over podocytes is increased in the solitary kidney. Nephrology Dialysis Transplantation, 29(1), 65-72. https://doi.org/10.1093/ndt/gft387

Fluid flow shear stress over podocytes is increased in the solitary kidney. / Srivastava, Tarak; Celsi, Gianni E.; Sharma, Mukut; Dai, Hongying; McCarthy, Ellen T.; Ruiz, Melanie; Cudmore, Patricia A.; Alon, Uri S.; Sharma, Ram; Savin, Virginia A.

In: Nephrology Dialysis Transplantation, Vol. 29, No. 1, 27.01.2014, p. 65-72.

Research output: Contribution to journalArticle

Srivastava, T, Celsi, GE, Sharma, M, Dai, H, McCarthy, ET, Ruiz, M, Cudmore, PA, Alon, US, Sharma, R & Savin, VA 2014, 'Fluid flow shear stress over podocytes is increased in the solitary kidney', Nephrology Dialysis Transplantation, vol. 29, no. 1, pp. 65-72. https://doi.org/10.1093/ndt/gft387
Srivastava, Tarak ; Celsi, Gianni E. ; Sharma, Mukut ; Dai, Hongying ; McCarthy, Ellen T. ; Ruiz, Melanie ; Cudmore, Patricia A. ; Alon, Uri S. ; Sharma, Ram ; Savin, Virginia A. / Fluid flow shear stress over podocytes is increased in the solitary kidney. In: Nephrology Dialysis Transplantation. 2014 ; Vol. 29, No. 1. pp. 65-72.
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abstract = "BackgroundGlomerular hyperfiltration is emerging as the key risk factor for progression of chronic kidney disease (CKD). Podocytes are exposed to fluid flow shear stress (FFSS) caused by the flow of ultrafiltrate within Bowman's space. The mechanism of hyperfiltration-induced podocyte injury is not clear. We postulated that glomerular hyperfiltration in solitary kidney increases FFSS over podocytes.MethodsInfant Sprague-Dawley rats at 5 days of age and C57BL/6J 14-week-old adult mice underwent unilateral nephrectomy. Micropuncture and morphological studies were then performed on 20-and 60-day-old rats. FFSS over podocytes in uninephrectomized rats and mice was calculated using the recently published equation by Friedrich et al. which includes the variables-single nephron glomerular filtration rate (SNGFR), filtration fraction (f), glomerular tuft diameter (2RT) and width of Bowman's space (s).ResultsGlomerular hypertrophy was observed in uninephrectomized rats and mice. Uninephrectomized rats on Day 20 showed a 2.0-fold increase in SNGFR, 1.0-fold increase in 2R T and 2.1-fold increase in FFSS, and on Day 60 showed a 1.9-fold increase in SNGFR, 1.3-fold increase in 2RT and 1.5-fold increase in FFSS, at all values of modeled 's'. Similarly, uninephrectomized mice showed a 2-to 3-fold increase in FFSS at all values of modeled SNGFR.ConclusionsFFSS over podocytes is increased in solitary kidneys in both infant rats and adult mice. This increase is a consequence of increased SNGFR. We speculate that increased FFSS caused by reduced nephron number contributes to podocyte injury and promotes the progression of CKD.",
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AU - Celsi, Gianni E.

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AU - Dai, Hongying

AU - McCarthy, Ellen T.

AU - Ruiz, Melanie

AU - Cudmore, Patricia A.

AU - Alon, Uri S.

AU - Sharma, Ram

AU - Savin, Virginia A.

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N2 - BackgroundGlomerular hyperfiltration is emerging as the key risk factor for progression of chronic kidney disease (CKD). Podocytes are exposed to fluid flow shear stress (FFSS) caused by the flow of ultrafiltrate within Bowman's space. The mechanism of hyperfiltration-induced podocyte injury is not clear. We postulated that glomerular hyperfiltration in solitary kidney increases FFSS over podocytes.MethodsInfant Sprague-Dawley rats at 5 days of age and C57BL/6J 14-week-old adult mice underwent unilateral nephrectomy. Micropuncture and morphological studies were then performed on 20-and 60-day-old rats. FFSS over podocytes in uninephrectomized rats and mice was calculated using the recently published equation by Friedrich et al. which includes the variables-single nephron glomerular filtration rate (SNGFR), filtration fraction (f), glomerular tuft diameter (2RT) and width of Bowman's space (s).ResultsGlomerular hypertrophy was observed in uninephrectomized rats and mice. Uninephrectomized rats on Day 20 showed a 2.0-fold increase in SNGFR, 1.0-fold increase in 2R T and 2.1-fold increase in FFSS, and on Day 60 showed a 1.9-fold increase in SNGFR, 1.3-fold increase in 2RT and 1.5-fold increase in FFSS, at all values of modeled 's'. Similarly, uninephrectomized mice showed a 2-to 3-fold increase in FFSS at all values of modeled SNGFR.ConclusionsFFSS over podocytes is increased in solitary kidneys in both infant rats and adult mice. This increase is a consequence of increased SNGFR. We speculate that increased FFSS caused by reduced nephron number contributes to podocyte injury and promotes the progression of CKD.

AB - BackgroundGlomerular hyperfiltration is emerging as the key risk factor for progression of chronic kidney disease (CKD). Podocytes are exposed to fluid flow shear stress (FFSS) caused by the flow of ultrafiltrate within Bowman's space. The mechanism of hyperfiltration-induced podocyte injury is not clear. We postulated that glomerular hyperfiltration in solitary kidney increases FFSS over podocytes.MethodsInfant Sprague-Dawley rats at 5 days of age and C57BL/6J 14-week-old adult mice underwent unilateral nephrectomy. Micropuncture and morphological studies were then performed on 20-and 60-day-old rats. FFSS over podocytes in uninephrectomized rats and mice was calculated using the recently published equation by Friedrich et al. which includes the variables-single nephron glomerular filtration rate (SNGFR), filtration fraction (f), glomerular tuft diameter (2RT) and width of Bowman's space (s).ResultsGlomerular hypertrophy was observed in uninephrectomized rats and mice. Uninephrectomized rats on Day 20 showed a 2.0-fold increase in SNGFR, 1.0-fold increase in 2R T and 2.1-fold increase in FFSS, and on Day 60 showed a 1.9-fold increase in SNGFR, 1.3-fold increase in 2RT and 1.5-fold increase in FFSS, at all values of modeled 's'. Similarly, uninephrectomized mice showed a 2-to 3-fold increase in FFSS at all values of modeled SNGFR.ConclusionsFFSS over podocytes is increased in solitary kidneys in both infant rats and adult mice. This increase is a consequence of increased SNGFR. We speculate that increased FFSS caused by reduced nephron number contributes to podocyte injury and promotes the progression of CKD.

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